2. Recombinant Proteins
  3. Complement System
  4. Complement Regulatory Proteins
  5. Complement Factor H Related 2
  6. CFHR2 Protein, Human (HEK293, His)

CFHR2, a key complement regulator, competently rivals CFH by avidly interacting with tissue-bound complement fragments. It also associates with lipoproteins, hinting at a role in lipid metabolism. With a distinctive head-to-tail homodimeric structure, CFHR2 forms heterodimers with CFHR1 or CFHR5, showcasing its versatile involvement in the regulatory dynamics of the complement system. CFHR2 Protein, Human (HEK293, His) is the recombinant human-derived CFHR2 protein, expressed by HEK293 , with C-6*His labeled tag.

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10 μg USD 115 In-stock
50 μg USD 345 In-stock
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Description

CFHR2, a key complement regulator, competently rivals CFH by avidly interacting with tissue-bound complement fragments. It also associates with lipoproteins, hinting at a role in lipid metabolism. With a distinctive head-to-tail homodimeric structure, CFHR2 forms heterodimers with CFHR1 or CFHR5, showcasing its versatile involvement in the regulatory dynamics of the complement system. CFHR2 Protein, Human (HEK293, His) is the recombinant human-derived CFHR2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background

CFHR2 takes center stage in the intricate landscape of complement regulation. Functioning as a crucial player, it engages in avid interactions with tissue-bound complement fragments, showcasing its ability to competently rival the physiological complement inhibitor CFH. Moreover, CFHR2 demonstrates a propensity to associate with lipoproteins, suggesting a potential involvement in lipid metabolism. Adding to its complexity, CFHR2 exhibits a distinctive head-to-tail homodimeric structure and forms heterodimers with CFHR1 or CFHR5, underscoring its versatile role in the regulatory ballet of the complement system.
Species

Human
Source

HEK293
Tag

C-6*His
Accession

P36980-1 (E19-K270)
Gene ID
Molecular Construction
N-term
CFHR2 (E19-K270)
Accession # P36980-1
6*His
C-term
Synonyms
rHuComplement factor H-related protein 2/CFHR2, His; Complement Factor H-Related Protein 2; FHR-2; DDESK59; H Factor-Like 3; H Factor-Like Protein 2; CFHR2; CFHL2; FHR2; HFL3
AA Sequence

EAMFCDFPKINHGILYDEEKYKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCAEEGWSPTPKCLRLCFFPFVENGHSESSGQTHLEGDTVQIICNTGYRLQNNENNISCVERGWSTPPKCRSTISAEKCGPPPPIDNGDITSFLLSVYAPGSSVEYQCQNLYQLEGNNQITCRNGQWSEPPKCLDPCVISQEIMEKYNIKLKWTNQQKLYSRTGDIVEFVCKSGYHPTKSHSFRAMCQNGKLVYPSCEEK

Molecular Weight

31&(33-36) kDa
Purity

Greater than 95% as determined by reducing SDS-PAGE
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, 2 mM EDTA, pH 7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation
Data Sheet (236 KB) SDS (252 KB)

COA (220 KB)

Handling Instructions (2659 KB)

CFHR2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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