2. Recombinant Proteins
  3. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  4. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  5. Eph Receptors
  6. EphA4
  7. EphA4 Protein, Human (HEK293, His-Fc)

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293, His-Fc) is the recombinant human-derived EphA4 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock
10 μg USD 68 Ask For Quote & Lead Time
50 μg USD 190 Ask For Quote & Lead Time
100 μg USD 300 Ask For Quote & Lead Time

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (HEK293, His-Fc) is the recombinant human-derived EphA4 protein, expressed by HEK293 , with C-hFc, C-His labeled tag.
Background

The EphA4 protein is a receptor tyrosine kinase that interacts with membrane-bound ephrin ligands on adjacent cells, enabling bidirectional signaling. It can be activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands, such as EFNA1 and EFNB3. Activation of EphA4 modulates cell morphology and integrin-dependent cell adhesion by regulating the activity of Rac, Rap, and Rho GTPases. In the nervous system, EphA4 plays a crucial role in axonal guidance, including corticospinal projections and segregation of motor and sensory axons during neuromuscular circuit development. It also contributes to synaptic plasticity and is involved in repair after injury, preventing axonal regeneration. EphA4's promiscuity allows it to participate in various cell-cell signaling processes, including the development of the thymic epithelium. Furthermore, it regulates pillar cell separation in the cochlear organ of Corti by forming a complex with ADAM10 and CADH1, leading to the disruption of adherens junctions. EphA4 phosphorylates CAPRIN1, promoting the formation of a membraneless compartment.
Species

Human
Source

HEK293
Tag

C-hFc;C-His
Accession

P54764-1/NP_004429.1 (V20-T547)
Gene ID
Molecular Construction
N-term
EphA4 (V20-T547)
Accession # P54764-1/NP_004429.1
hFc-His
C-term
Synonyms
Ephrin type-A receptor 4; EPH-like kinase 8; EK8; EPHA4; HEK8; SEK; TYRO1
AA Sequence

MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST

Molecular Weight

100-110 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Data Sheet (238 KB) Handling Instructions (2659 KB)

EphA4 Protein, Human (HEK293, His-Fc) Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
EphA4 Protein, Human (HEK293, His-Fc)
Cat. No.:
HY-P72994
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (0 KB) Français - FR (0 KB) Deutsch - DE (0 KB) Norwegian - NO (0 KB) Español - ES (0 KB) Swedish - SV (0 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.