Granzyme B/GZMB Protein, Mouse (HEK293, C-His)

Granzyme B/GZMB Protein, found in cytotoxic T-cells and NK-cells, activates caspase-independent pyroptosis and cleaves gasdermin-E (GSDME), inducing target cell death. It also participates in caspase activation cascade, promoting apoptosis and plasma membrane repair during bacterial infection. Granzyme B/GZMB Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived Granzyme B/GZMB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Granzyme B/GZMB Protein, Mouse (HEK293, C-His) is 229 a.a., with molecular weight of ~35.0 kDa.

Granzyme B/GZMB Protein, an abundant protease found in the cytosolic granules of cytotoxic T-cells and NK-cells, plays a crucial role in various cellular processes. When delivered into the target cell through the immunological synapse, Granzyme B/GZMB activates caspase-independent pyroptosis, leading to target cell death. It achieves this by cleaving after Asp and catalyzing the cleavage of gasdermin-E (GSDME), releasing the pore-forming component of GSDME, which triggers pyroptosis. Granzyme B/GZMB is also involved in the activation cascade of caspases, including caspase-3, -9, and -7, which are responsible for apoptosis execution and plasma membrane repair in response to bacterial infection.

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'-Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is 2356 pmol/min/µg as measured under the described conditions.

Mouse

HEK293

C-6*His

P04187 (G19-S247)

14939

GEIIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Approximately 35.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.