Grb2 Protein, Human (His)

Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag.

Grb2, an adapter protein, plays a pivotal role in establishing a crucial connection between cell surface growth factor receptors and the Ras signaling pathway. Despite its lack of direct binding to phosphorylated epidermal growth factor receptor (EGFR), Grb2 exerts an inhibitory effect on EGF-induced transactivation of a RAS-responsive element. Notably, Grb2 functions as a dominant negative protein relative to GRB2, suppressing proliferative signals and potentially instigating active programmed cell death.

1.Immobilized Human GRB2 at 2 μg/mL (100 μL/well) can bind Anti-GRB2 Antibody. The ED₅₀ for this effect is 93.39 ng/mL.
2.Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is ≤33.3 ng/mL, corresponding to a specific activity is 3.003×10⁴ units/mg

• 
  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 33.3 ng/mL, corresponding to a specific activity is 3.003×10⁴ units/mg.

Human

E. coli

C-6*His

P62993-1 (M1-V217)

2885  [NCBI]

MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Approximately 25-29 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 8.0 or 50 mM Tris-HCL, 150 mM NaCl, pH 8.0, 8% trehalose, 0.02% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.