HNF4A Protein, Human (His)

HNF 4 alpha, a nuclear transcription factor, forms homodimers to bind DNA. It regulates gene expression, including hepatocyte nuclear factor 1 alpha, impacting hepatic genes. Its involvement extends to liver, kidney, and intestinal development. Mutations associate with autosomal dominant non-insulin-dependent diabetes mellitus type I. The gene exhibits biased expression in the duodenum (RPKM 37.7) and colon (RPKM 32.8), among other tissues. HNF4A Protein, Human (His) is the recombinant human-derived HNF 4 alpha, expressed by HEK293 , with C-10*His labeled tag.

HNF 4 alpha, the protein product of this gene, functions as a nuclear transcription factor, forming homodimers to bind DNA and exerting control over the expression of various genes, including hepatocyte nuclear factor 1 alpha, a key regulator of hepatic gene expression. Its involvement extends to the development of vital organs such as the liver, kidney, and intestines. Mutations in HNF 4 alpha have been linked to monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I, underscoring its significance in metabolic regulation. Additionally, alternative splicing yields multiple transcript variants, generating diverse isoforms. Notably, HNF 4 alpha displays biased expression, with higher levels observed in the duodenum (RPKM 37.7), colon (RPKM 32.8), and seven other tissues, emphasizing its specialized role in diverse physiological contexts.

Human

HEK293

C-10*His

P41235-1/NP_000448 (M1-I474)

3172

MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI

Approximately 70-80 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 10% glycerol, pH 7.3.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.