IL-12R beta 2 Protein, Mouse (HEK293, Fc)

IL-12R beta 2 protein is an IL-12 cytokine surface receptor that activates the Jak-Stat signaling cascade pathway and is involved in IL-12-mediated immune regulation. IL-12R beta 2 Protein, Mouse (HEK293, Fc) is expressed by HEK293 cells with a hFc tag at the C-terminus^[1].

IL-12 receptor is a type I cytokine receptor that binds to IL-12. IL-12 receptor is expressed primarily on NK and T cells and consists of beta 1 and beta 2 subunits and is a member of the gp130 cytokine receptor superfamily. IL-12R beta 2 (abbreviated IL-12R2 or IL-12Rβ2) is a subunit of the IL-12 receptor, and IL-12Rβ2 is its human gene name. Co-expression of IL-12Rβ2 protein and IL-12Rβ1 protein can lead to the formation of high-affinity IL-12 binding sites and the reconstitution of IL-12-dependent signaling. Upon IL-12 binding to the IL-12 receptor, the cytoplasmic protein TYK2, which interacts directly with IL-12Rβ1, and JAK2, which interacts with IL-12Rβ2, are tyrosine phosphorylated. Phosphorylated TYK2 and JAK2 are required for subsequent tyrosine phosphorylation and activation of STAT4 that binds to IL-12Rβ2. STAT4 is a transcription factor that subsequently homodimerizes, translocates to the nucleus and binds to its target DNA to activate transcription of IFN-γ and other target genes^[1].
Expression of the IL-12Rβ2 chain is thought to be a key factor in controlling T cell development and function. It plays an important role in Th1 cell differentiation and deletion results in failure of Th1 differentiation and dysfunctional production of Th1 effector molecules. Up-regulation of this gene has been found to be associated with many infectious diseases, such as Crohn's disease and leprosy, which are thought to contribute to the inflammatory response and host defense^[2].

The number of Treg cells in the resting spleen of IL-12Rβ2^-/- mice is comparable to that of strain-matched wild-type (WT) mice. Among them, IL-12Rβ2^-/- splenocytes are completely resistant to IL-12-mediated inhibition and lacked detectable IL-12 receptor mRNA in reg cells, suggesting that IL-12 signaling can indirectly inhibit Treg amplification^[3].
IL-12Rβ2 is expressed primarily in NK cells, and mice lacking the IL-12Rβ2 subunit exhibit transient weight loss, the severity of which correlates with serum cytokine levels of IFN-γ. And, serum levels of IL-1β and IL-23 can be detected in IL-12Rβ^-/-.Rag1^-/-mice on C57BL/6 background, even in the absence of anti-CD40 treatment. IL-12Rβ2^-/- .Rag1^-/- mice exhibits elevated mRNA levels of most pro-inflammatory cytokines^[4].

Mouse

HEK293

C-hFc

P97378 (N24-N637)

16162  [NCBI]

MAQTVRECSLALLFLFMWLLIKANIDVCKLGTVTVQPAPVIPLGSAANISCSLNPKQGCSHYPSSNELILLKFVNDVLVENLHGKKVHDHTGHSSTFQVTNLSLGMTLFVCKLNCSNSQKKPPVPVCGVEISVGVAPEPPQNISCVQEGENGTVACSWNSGKVTYLKTNYTLQLSGPNNLTCQKQCFSDNRQNCNRLDLGINLSPDLAESRFIVRVTAINDLGNSSSLPHTFTFLDIVIPLPPWDIRINFLNASGSRGTLQWEDEGQVVLNQLRYQPLNSTSWNMVNATNAKGKYDLRDLRPFTEYEFQISSKLHLSGGSWSNWSESLRTRTPEEEPVGILDIWYMKQDIDYDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTSWTRVIPRTGAWTASVSAANSKGASAPTHINIVDLCGTGLLAPHQVSAKSENMDNILVTWQPPKKADSAVREYIVEWRALQPGSITKFPPHWLRIPPDNMSALISENIKPYICYEIRVHALSESQGGCSSIRGDSKHKAPVSGPHITAITEKKERLFISWTHIPFPEQRGCILHYRIYWKERDSTAQPELCEIQYRRSQNSHPISSLQPRVTYVLWMTAVTAAGESPQGNEREFCPQGKAN

125-160 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Esther van de Vosse, et al. IL-12Rβ1 deficiency: mutation update and description of the IL12RB1 variation database. Hum Mutat. 2013 Oct;34(10):1329-39.  [Content Brief]

  [2]. Ryuta Nishikomori, et al. Activated STAT4 has an essential role in Th1 differentiation and proliferation that is independent of its role in the maintenance of IL-12R beta 2 chain expression and signaling. J Immunol. 2002 Oct 15;169(8):4388-98.  [Content Brief]

  [3]. Xuefang Cao, et al. Interleukin 12 stimulates IFN-gamma-mediated inhibition of tumor-induced regulatory T-cell proliferation and enhances tumor clearance. Cancer Res. 2009 Nov 15;69(22):8700-9.  [Content Brief]

  [4]. Gaëlle Chognard, et al. The dichotomous pattern of IL-12r and IL-23R expression elucidates the role of IL-12 and IL-23 in inflammation. PLoS One. 2014 Feb 21;9(2):e89092.  [Content Brief]