IL-2R alpha/CD25 Protein, Human (HEK293, C-His)

IL-2R alpha protein is the receptor for interleukin 2 and plays a crucial role in immune tolerance by regulating the activity of regulatory T cells (TREG). These TREGs are responsible for inhibiting the activation and expansion of autoreactive T cells.IL-2R alpha/CD25 Protein, Human (HEK293, C-His) is the recombinant human-derived IL-2R alpha protein, expressed by HEK293 , with C-6*His labeled tag.

IL-2R alpha, a receptor for interleukin-2, plays a pivotal role in the regulation of immune tolerance by modulating the activity of regulatory T cells (TREGs). TREGs function to suppress the activation and expansion of autoreactive T-cells, contributing to immune homeostasis. The receptor exists in three distinct forms: a high-affinity dimer, an intermediate-affinity monomer (beta subunit), and a low-affinity monomer (alpha subunit). Both high and intermediate affinity forms can associate with a gamma subunit, highlighting the multifaceted nature of IL-2R in mediating immune responses.

2 μg/mL (100 μL/well) of immobilized Human IL-2RA-His can bind Human IL-2-His-Avi with an ED₅₀ value of 4.07 ng/mL.

Human

HEK293

C-6*His

P01589 (E22-C213)

3559  [NCBI]

ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

Approximately 44.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 10 mM HEPES, 50 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.