KIRREL2/NEPH3 Protein, Human (HEK293, Fc)

KIRREL2/NEPH3 proteins may regulate basal insulin secretion and participate in self-association as homodimers. Functionally, it interacts with NPHS2/podocin, NPHS1 and FYN, contributing to key molecular interactions in cellular processes. KIRREL2/NEPH3 Protein, Human (HEK293, Fc) is the recombinant human-derived KIRREL2/NEPH3 protein, expressed by HEK293 , with C-hFc labeled tag.

KIRREL2/NEPH3 Protein is implicated in potentially regulating basal insulin secretion, suggesting its involvement in the intricate processes of insulin release. Structurally, it forms homodimers, indicative of its ability to engage in self-association. Functionally, KIRREL2/NEPH3 interacts with key proteins such as NPHS2/podocin, NPHS1, and FYN, highlighting its involvement in molecular interactions crucial for cellular processes. The interaction with NPHS2/podocin occurs through the C-terminus, while the interaction with NPHS1 involves the Ig-like domains, suggesting diverse binding mechanisms. Furthermore, the association with FYN adds a layer of complexity to its potential roles in cellular signaling pathways. The multifaceted interactions and potential regulatory roles of KIRREL2/NEPH3 underscore its significance in cellular functions beyond insulin secretion, warranting further investigation to unravel its precise contributions to various biological processes.

Human

HEK293

C-hFc

Q6UWL6-1 (G21-R503)

84063  [NCBI]

GPSPHFLQQPEDLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAGLRSRPAQLHVLVPPEAPQVLGGPSVSLVAGVPANLTCRSRGDARPTPELLWFRDGVLLDGATFHQTLLKEGTPGSVESTLTLTPFSHDDGATFVCRARSQALPTGRDTAITLSLQYPPEVTLSASPHTVQEGEKVIFLCQATAQPPVTGYRWAKGGSPVLGARGPRLEVVADASFLTEPVSCEVSNAVGSANRSTALDVLFGPILQAKPEPVSVDVGEDASFSCAWRGNPLPRVTWTRRGGAQVLGSGATLRLPSVGPEDAGDYVCRAEAGLSGLRGGAAEARLTVNAPPVVTALHSAPAFLRGPARLQCLVFASPAPDAVVWSWDEGFLEAGSQGRFLVETFPAPESRGGLGPGLISVLHISGTQESDFSRSFNCSARNRLGEGGAQASLGRR

Approximately 85-95 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.