Lon Protease Protein, E. coli (His)

Lon Protease, an ATP-dependent serine protease, selectively degrades mutant proteins, short-lived regulators, and specific antitoxins, vital for cellular homeostasis. Crucial for survival during DNA damage and stress-induced changes, it processively degrades polypeptides into 5-10 amino acid fragments. Lon Protease also exhibits site-specific, double-stranded DNA binding. Its substrates include RcsA, SulA, SoxS, UmuD, and type II antitoxins like CcdA, HipB, and MazE. Notably, overproduced Lon Protease selectively hinders translation through pathways, including the YoeB-YefM toxin-antitoxin system. Lon Protease Protein, E. coli (His) is the recombinant E. coli-derived Lon Protease protein, expressed by E. coli, with C-His labeled tag. The total length of Lon Protease Protein, E. coli (His) is 784 a.a., with molecular weight of ~88 kDa.

Lon Protease, an ATP-dependent serine protease, serves as a crucial mediator in the selective degradation of mutant and aberrant proteins, as well as certain short-lived regulatory proteins, including specific antitoxins. Essential for cellular homeostasis, Lon Protease plays a pivotal role in ensuring survival under conditions of DNA damage and stress-induced developmental changes. Operating processively, this protease degrades polypeptides to generate small peptide fragments typically ranging from 5 to 10 amino acids in length. Additionally, Lon Protease exhibits a DNA-binding capacity in a double-stranded, site-specific manner. Among its endogenous substrates are regulatory proteins like RcsA, SulA, SoxS, UmuD, and various type II antitoxins such as CcdA, HipB, and MazE. Notably, Lon Protease, when overproduced, selectively impedes translation through distinct pathways, including the YoeB-YefM toxin-antitoxin system.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

C-His

P0A9M0 (M1-K784)

61752336

Approximately 88 kDa

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.