PCSK9 Protein, Mouse (HEK293, His, solution)

The PCSK9 protein regulates cholesterol levels by degrading LDLR, VLDLR, LRP1/APOER, and LRP8/APOER2 receptors. It promotes LDLR degradation, inhibits recycling, induces ubiquitination, and affects APOB degradation. Additionally, PCSK9 influences BACE1 intermediates, ENaC surface expression, and neuronal apoptosis by modulating LRP8/APOER2 levels and anti-apoptotic signaling pathways. PCSK9 Protein, Mouse (HEK293, His, solution) is a recombinant protein dimer complex containing mouse-derived PCSK9 protein, expressed by HEK293 , with C-6*His labeled tag. PCSK9 Protein, Mouse (HEK293, His, solution), has molecular weight of 17-19 & 60-66 kDa, respectively.

The PCSK9 protein plays a pivotal role in the intricate regulation of plasma cholesterol homeostasis. It engages with members of the low-density lipid receptor family, including low-density lipoprotein receptor (LDLR), very low-density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER), and apolipoprotein receptor 2 (LRP8/APOER2), facilitating their degradation within intracellular acidic compartments. Operating through a non-proteolytic mechanism, PCSK9 enhances the degradation of hepatic LDLR via a clathrin LDLRAP1/ARH-mediated pathway and may impede LDLR recycling from endosomes to the cell surface, directing it to lysosomes for degradation. Moreover, PCSK9 induces ubiquitination of LDLR, leading to subsequent degradation, and inhibits the intracellular degradation of APOB through the autophagosome/lysosome pathway, independent of LDLR. Additionally, PCSK9 is implicated in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway, hinders epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by increasing its proteasomal degradation, and modulates neuronal apoptosis by regulating LRP8/APOER2 levels and related anti-apoptotic signaling pathways.

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its binding ability in a functional ELISA. When Recombinant Mouse LDLR Protein is immobilized at 2 µg/mL (100 µL/well) can bind Mouse PCSK9 Protein. The ED₅₀ for this effect is 145.3ng/mL.

Mouse

HEK293

C-6*His

Q80W65 (Q35-Q155&S156-Q694)

100102  [NCBI]

QDEDGDYEELMLALPSQEDGLADEAAHVATATFRRCSKEAWRLPGTYIVVLMEETQRLQIEQTAHRLQTRAARRGYVIKVLHIFYDLFPGFLVKMSSDLLGLALKLPHVEYIEEDSFVFAQSIPWNLERIIPAWHQTEEDRSPDGSSQVEVYLLDTSIQGAHREIEGRVTITDFNSVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGTSLHSLRVLNCQGKGTVSGTLIGLEFIRKSQLIQPSGPLVVLLPLAGGYSRILNAACRHLARTGVVLVAAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGKDIIGASSDCSTCFMSQSGTSQAAAHVAGIVARMLSREPTLTLAELRQRLIHFSTKDVINMAWFPEDQQVLTPNLVATLPPSTHETGGQLLCRTVWSAHSGPTRTATATARCAPEEELLSCSSFSRSGRRRGDWIEAIGGQQVCKALNAFGGEGVYAVARCCLVPRANCSIHNTPAARAGLETHVHCHQKDHVLTGCSFHWEVEDLSVRRQPALRSRRQPGQCVGHQAASVYASCCHAPGLECKIKEHGISGPSEQVTVACEAGWTLTGCNVLPGASLTLGAYSVDNLCVARVHDTARADRTSGEATVAAAICCRSRPSAKASWVQ

17-19 & 60-66 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 20% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.