PLK1 Protein, Mouse (sf9, His)

PLK1 Protein, the subject of the paragraph, is not mentioned in the provided text.If you would like a summary involving PLK1, please provide the relevant paragraph or information about PLK1.PLK1 Protein, Mouse (sf9, His) is the recombinant mouse-derived PLK1 protein, expressed by Sf9 insect cells, with N-His labeled tag.

The protein SGO1 plays a role in regulating the cohesion of centrioles. It phosphorylates FBXO5/EMI1, a negative regulator of the APC/C complex, leading to its degradation. It also acts as a negative regulator of p53 family members, phosphorylating TOPORS to inhibit the sumoylation of p53/TP53 and enhance its ubiquitination and degradation. SGO1 phosphorylates the transactivation domain of the transcription factor p73/TP73, inhibiting its transcriptional activation and pro-apoptotic functions. It also phosphorylates BORA, promoting its degradation. SGO1 is involved in the regulation of AURKA function, DNA damage checkpoint recovery, and entry into mitosis. It phosphorylates MISP, stabilizing microtubule attachments required for proper spindle positioning. In meiosis, SGO1, along with MEIKIN, regulates kinetochore function and protects centromeric cohesin from separase-mediated cleavage. SGO1 phosphorylates CEP68 and promotes its degradation. It also phosphorylates DCTN1, regulating nuclear envelope breakdown during prophase. SGO1 phosphorylates the heat shock transcription factor HSF1, promoting its nuclear translocation in response to heat shock. In early mitotic period, SGO1 phosphorylates HSF1, regulating its localization to the spindle pole and subsequent degradation. SGO1 phosphorylates RIOK2, regulating mitotic progression. Finally, SGO1 phosphorylates DZIP1, regulating the localization of the BBSome during mitosis, a protein complex involved in cilium biogenesis.

Mouse

Sf9 insect cells

N-His

Q07832 (M1-S603)

18817  [NCBI]

Approximately 65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.