Transferrin Protein, Human (HEK293, His)

Transferrin proteins, iron-binding transporters, bind two Fe(3+) ions and an anion, usually bicarbonate. They transport iron from absorption and heme degradation sites to storage and utilization locations. Serum transferrin may impact cell proliferation. In microbial infection, Neisseria species utilize transferrin as an iron source, capturing and extracting iron for their metabolic needs. Transferrin Protein, Human (HEK293, His) is the recombinant human-derived Transferrin protein, expressed by HEK293 , with C-His labeled tag. The total length of Transferrin Protein, Human (HEK293, His) is 679 a.a., with molecular weight of 68-87 kDa.

Transferrins, as iron-binding transport proteins, have the capacity to bind two Fe(3+) ions in conjunction with an anion, typically bicarbonate. They play a pivotal role in transporting iron from sites of absorption and heme degradation to locations designated for storage and utilization. Additionally, serum transferrin may exert influence over cell proliferation. In the context of microbial infection, transferrin serves as an iron source for Neisseria species, which adeptly capture the protein and extract iron for their own metabolic needs.

1.Measured by its binding ability in a functional ELISA. Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R. The ED₅₀ for this effect is 10.21 ng/mL.
2. Measured in a cell proliferation assay using HepG2 Human Hepatocellular Carcinoma Cells. The ED₅₀ for this effect is 21.49 μg/mL, corresponding to a spec ific activity is 46.533 U/mg.
3.Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.1817 μg/mL, corresponding to a specific activity is 5503.58 units/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Human Transferrin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Transferrin R,The ED₅₀  for this effect is 10.21 ng/mL

Human

HEK293

C-His

P02787/AAA61140.1/NP_001054.1 (V20-P698)

7018  [NCBI]

VPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP

68-87 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.