uPAR Protein, Mouse (Biotinylated, HEK293, His-Avi)

The uPAR protein acts as a receptor for urokinase plasminogen activator, facilitating plasmin formation and signal transduction activation. It interacts with SRPX2 and forms a monomer. uPAR also interacts with MRC2 and SORL1, reducing PLAUR internalization. Additionally, the PLAUR-PLAU-SERPINE1 complex interacts with SORL1. uPAR Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived uPAR protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of uPAR Protein, Mouse (Biotinylated, HEK293, His-Avi) is 275 a.a., with molecular weight of 50-70 kDa.

The uPAR protein functions as a receptor for urokinase plasminogen activator and plays a crucial role in localizing and facilitating the formation of plasmin. Additionally, it mediates signal transduction activation effects of U-PA independently of proteolysis. It is predominantly found as a monomer and interacts with SRPX2 through its UPAR/Ly6 domains. uPAR also interacts with MRC2 and SORL1, with the latter interaction reducing PLAUR internalization. Moreover, the ternary complex consisting of PLAUR-PLAU-SERPINE1 also interacts with SORL1.

Human PLAU, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse uPAR, His Tag with an affinity constant of 418.50 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-Avi;C-His

P35456-1 (L24-G298)

/

50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.