RNase A, Bovine pancreas  (Synonyms: Ribonuclease A; EC 4.6.1.18; RNase A)

RNase A, Bovine pancreas (Ribonuclease A) cleaves the 3' end of RNA to pyrimidine and actively cleaves RNA at each pyrimidine residue. RNase A, bovine pancreas catalyzes the hydrolysis of single-stranded RNA in the absence of metal ions or cofactors^[1][2][3].

A major application of RNase A is the removal of RNA from plasmid DNA preparations.

Preparation of RNase A stock solution
1. Use 10 mM sodium acetate (pH 5.2) to prepare 10 mg/mL RNase A stock solution;
2. Heat at 100 °C for 15 min;
3. Cool to room temperature, add 1/10 (v/v) of 1 M Tris-HCl (pH 7.4), adjust the pH to 7.4;
4. The solution can be stored at 20 °C for 2 years.
Note: When the RNase A solution is boiled under neutral conditions, RNase precipitate will form; if it is boiled at a lower pH, any precipitation may be caused by the presence of protein impurities. At this time, the precipitate can be removed by high-speed centrifugation (13 000 rpm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-99-4

Solid

White to off-white

[RNase A, Bovine pancreas]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Thakur P, et al. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis. Methods Enzymol. 2021;658:1-24.  [Content Brief]

  [2]. Brown D, Pasloske BL. Ribonuclease-resistant RNA controls and standards. Methods Enzymol. 2001;341:648-54.  [Content Brief]

  [3]. Wallace JA, Shen JK. Predicting pKa values with continuous constant pH molecular dynamics. Methods Enzymol. 2009;466:455-75.  [Content Brief]