RL648_81

Name: RL648_81
Brand: MedChemExpress
SKU: HY-123264
Rating: 4.5 (1 reviews)

Cat. No.: HY-123264 Purity: 99.20%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

RL648_81 is a specific KQT-like subfamily 2/3 (KCNQ2/3) activator with an EC₅₀ of 190 nM. RL648_81 robustly shifts the V1/2 of KCNQ2/3 channels towards hyperpolarized potentials.RL648_81 does not shift the V1/2 of either KCNQ4 or KCNQ5.RL648_81 has the potential for neurologic disorders associated with neuronal hyperexcitability research.

For research use only. We do not sell to patients.

RL648_81 Chemical Structure

CAS No. : 1919050-87-5

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 143	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 143	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	190 nM Compound: RL-81	Agonist activity at human Kv7.2/Kv7.3 expressed in CHO cells at holding potential of at -85 mV by whole cell patch clamp electrophysiology assay Agonist activity at human Kv7.2/Kv7.3 expressed in CHO cells at holding potential of at -85 mV by whole cell patch clamp electrophysiology assay	[PMID: 31223450]

Molecular Weight

371.33

Formula

C₁₇H₁₇F₄N₃O₂

CAS No.

1919050-87-5

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(NC1=CC=C(C(F)=C1N)NCC2=CC=C(C=C2)C(F)(F)F)OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (269.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6930 mL	13.4651 mL	26.9302 mL
5 mM	0.5386 mL	2.6930 mL	5.3860 mL
10 mM	0.2693 mL	1.3465 mL	2.6930 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.20%

Select Batch:

Data Sheet (269 KB) SDS (251 KB)

COA (245 KB) HNMR (273 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Manoj Kumar, et al. Synthesis and Evaluation of Potent KCNQ2/3-Specific Channel Activators. Mol Pharmacol. 2016 Jun;89(6):667-77. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6930 mL	13.4651 mL	26.9302 mL	67.3256 mL
	5 mM	0.5386 mL	2.6930 mL	5.3860 mL	13.4651 mL
	10 mM	0.2693 mL	1.3465 mL	2.6930 mL	6.7326 mL
	15 mM	0.1795 mL	0.8977 mL	1.7953 mL	4.4884 mL
	20 mM	0.1347 mL	0.6733 mL	1.3465 mL	3.3663 mL
	25 mM	0.1077 mL	0.5386 mL	1.0772 mL	2.6930 mL
	30 mM	0.0898 mL	0.4488 mL	0.8977 mL	2.2442 mL
	40 mM	0.0673 mL	0.3366 mL	0.6733 mL	1.6831 mL
	50 mM	0.0539 mL	0.2693 mL	0.5386 mL	1.3465 mL
	60 mM	0.0449 mL	0.2244 mL	0.4488 mL	1.1221 mL
	80 mM	0.0337 mL	0.1683 mL	0.3366 mL	0.8416 mL
	100 mM	0.0269 mL	0.1347 mL	0.2693 mL	0.6733 mL

RL648_81 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RL648_811919050-87-5Potassium ChannelKcsAKQT-like subfamilyKCNQ2/3V1/2hyperpolarized potentialsKCNQ4KCNQ5,?neurologic disorderhyperexcitabilityInhibitorinhibitorinhibit

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RL648_81

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Complete Stock Solution Preparation Table

RL648_81 Related Classifications

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