RN-1 dihydrochloride

Name: RN-1 dihydrochloride
Brand: MedChemExpress
SKU: HY-110130
Rating: 4.5 (1 reviews)

Cat. No.: HY-110130 Purity: 99.13%: FAQs
Data Sheet SDS COA Handling Instructions

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RN-1 dihydrochloride is a potent, brain-penetrant, irreversible and selective lysine-specific demethylase 1 (LSD1) inhibitor with an IC₅₀ of 70 nM. RN-1 dihydrochloride exhibits selectivity for LSD1 over MAO-A and MAO-B with IC₅₀ values of 0.51 μM and 2.785 μM respectively.

For research use only. We do not sell to patients.

RN-1 dihydrochloride Chemical Structure

CAS No. : 1781835-13-9

Size	Price	Stock	Quantity
5 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 195	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 485	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 690	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: 70 nM (LSD1), 0.51 μM (MAO-A) and 2.785 μM (MAO-B)^[1]

In Vitro

RN-1 dihydrochloride shows cytotoxic for ovarian cancer cells (SKOV3, OVCAR3, A2780 and cisplatin-resistant A2780cis), with IC50 values of ≈100-200 μM^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

RN-1 (3-10 mg/kg; i.p.; daily; for 2 or 4 consecutive weeks) dihydrochloride effectively induces fetal hemoglobin (HbF) levels in red blood cells and reduces disease pathology in SCD mice^[2].
In C57BL/6 male mice, after intraperitoneal administration of RN-1 dihydrochloride (10 mg/kg), concentrations are detectable up to 24 h post dose in both plasma and brain tissues. The brain/plasma exposure ratio is 88.9. RN-1 dihydrochloride significantly impairs long-term memory, but not short-term memory^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sickle cell disease (SCD) mice^[2]
Dosage:	3 mg/kg or 10 mg/kg
Administration:	i.p.; daily; for 2 or 4 consecutive weeks
Result:	Effectively induced HbF levels in red blood cells and reduced disease pathology in SCD mice.

Molecular Weight

452.42

Formula

C₂₃H₃₁Cl₂N₃O₂

CAS No.

1781835-13-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(C)CC1)CN[C@@H]2[C@@H](C3=CC=C(OCC4=CC=CC=C4)C=C3)C2.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 20 mg/mL (44.21 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2103 mL	11.0517 mL	22.1034 mL
5 mM	0.4421 mL	2.2103 mL	4.4207 mL
10 mM	0.2210 mL	1.1052 mL	2.2103 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 20 mg/mL (44.21 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.13%

Select Batch:

Data Sheet (271 KB) SDS (251 KB)

COA (245 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. Ramesh Neelamegam, et al. Brain-penetrant LSD1 inhibitors can block memory consolidation. ACS Chem Neurosci. 2012 Feb 15;3(2):120-128. [Content Brief]

[2]. Shuaiying Cui, et al. The LSD1 inhibitor RN-1 induces fetal hemoglobin synthesis and reduces disease pathology in sickle cell mice. Blood. 2015 Jul 16;126(3):386-96. [Content Brief]

[3]. Sergiy Konovalov, et al. Analysis of the levels of lysine-specific demethylase 1 (LSD1) mRNA in human ovarian tumors and the effects of chemical LSD1 inhibitors in ovarian cancer cell lines. J Ovarian Res. 2013 Oct 29;6(1):75. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.2103 mL	11.0517 mL	22.1034 mL	55.2584 mL
	5 mM	0.4421 mL	2.2103 mL	4.4207 mL	11.0517 mL
	10 mM	0.2210 mL	1.1052 mL	2.2103 mL	5.5258 mL
	15 mM	0.1474 mL	0.7368 mL	1.4736 mL	3.6839 mL
	20 mM	0.1105 mL	0.5526 mL	1.1052 mL	2.7629 mL
	25 mM	0.0884 mL	0.4421 mL	0.8841 mL	2.2103 mL
	30 mM	0.0737 mL	0.3684 mL	0.7368 mL	1.8419 mL
	40 mM	0.0553 mL	0.2763 mL	0.5526 mL	1.3815 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.