2. GPCR/G Protein Neuronal Signaling
  3. Melanocortin Receptor
  4. RO27-3225 TFA

RO27-3225 TFA is potent and selective melanocortin 4 receptor (MC4R) agonist with an EC50 of 1 nM and 8 nM for MC4R and MC1R, respectively. RO27-3225 TFA shows ~30-fold selectivity for MC4R over MC3R. RO27-3225 TFA has neuroprotective and anti-inflammatory effects.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

RO27-3225 TFA Chemical Structure

RO27-3225 TFA Chemical Structure

Size Price Stock Quantity
5 mg USD 300 In-stock
10 mg USD 480 In-stock
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100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

RO27-3225 TFA Related Antibodies

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View All Melanocortin Receptor Isoform Specific Products:

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MC1R MC3R MC2R MC4R MC5R

  • Biological Activity

  • Purity & Documentation

  • References

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Description

RO27-3225 TFA is potent and selective melanocortin 4 receptor (MC4R) agonist with an EC50 of 1 nM and 8 nM for MC4R and MC1R, respectively. RO27-3225 TFA shows ~30-fold selectivity for MC4R over MC3R. RO27-3225 TFA has neuroprotective and anti-inflammatory effects[1][2][3].

IC50 & Target

MC4R

 

In Vivo

RO27-3225 (0.012-0.048 mg/kg; intravenous injection; Wistar rats) treatment reverses haemorrhagic shock, reduces multiple organ damage and improves survival. RO27-3225 could have a protective role against multiple organ failure following circulatory shock[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar rats of both sexes (270-300 g) with haemorrhagic shock[2]
Dosage: 0.012 mg/kg, 0.024 mg/kg, 0.048 mg/kg
Administration: Intravenous injection
Result: Reversed haemorrhagic shock, reduced multiple organ damage and improved survival.
Molecular Weight

898.93

Formula

C41H53F3N12O8
Appearance

Solid

Color

Off-white to light yellow

Sequence

Oxobutyl-His-Phe-Arg-Trp-{Sar}-NH2
Sequence Shortening

Oxobutyl-HFRW-{Sar}-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (111.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 100 mg/mL (111.24 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1124 mL 5.5622 mL 11.1243 mL
5 mM 0.2225 mL 1.1124 mL 2.2249 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (2.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (2.78 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 100 mg/mL (111.24 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.1124 mL 5.5622 mL 11.1243 mL 27.8108 mL
5 mM 0.2225 mL 1.1124 mL 2.2249 mL 5.5622 mL
10 mM 0.1112 mL 0.5562 mL 1.1124 mL 2.7811 mL
15 mM 0.0742 mL 0.3708 mL 0.7416 mL 1.8541 mL
20 mM 0.0556 mL 0.2781 mL 0.5562 mL 1.3905 mL
25 mM 0.0445 mL 0.2225 mL 0.4450 mL 1.1124 mL
30 mM 0.0371 mL 0.1854 mL 0.3708 mL 0.9270 mL
40 mM 0.0278 mL 0.1391 mL 0.2781 mL 0.6953 mL
50 mM 0.0222 mL 0.1112 mL 0.2225 mL 0.5562 mL
60 mM 0.0185 mL 0.0927 mL 0.1854 mL 0.4635 mL
80 mM 0.0139 mL 0.0695 mL 0.1391 mL 0.3476 mL
100 mM 0.0111 mL 0.0556 mL 0.1112 mL 0.2781 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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RO27-3225 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RO27-3225Melanocortin ReceptorMC ReceptorMC4RMC1Ranti-inflammatoryauditoryfoodintakehemorrhagicshocksurvivalneuroprotectiveInhibitorinhibitorinhibit

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