Rotenolone

Name: Rotenolone
Brand: MedChemExpress
SKU: HY-119694
Rating: 4.5 (1 reviews)

Cat. No.: HY-119694 Purity: ≥97.0%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Rotenolone is an antiproliferative agent. Rotenolone shows antiproliferative activity against the ovarian cancer A2780, breast cancer BT-549, prostate cancer DU 145, NSCLC NCI-H460, and colon cancer HCC-2998 cell lines, with IC₅₀s of 0.95, 1.6, 2.7, 2.0, and 2.9 μM, respectively.

For research use only. We do not sell to patients.

Rotenolone Chemical Structure

CAS No. : 509-96-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
697	IC₅₀	0.5 μM Compound: 7	Cytotoxicity against human 697 cells after 72 hrs by MTS assay Cytotoxicity against human 697 cells after 72 hrs by MTS assay	[PMID: 23895019]
697	IC₅₀	0.7 μM Compound: 7	Cytotoxicity against human 697 cells after 48 hrs by MTS assay Cytotoxicity against human 697 cells after 48 hrs by MTS assay	[PMID: 23895019]
A2780	IC₅₀	0.95 μM Compound: 5	Antiproliferative activity against human A2780 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay Antiproliferative activity against human A2780 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 20804165]
BT-549	IC₅₀	1.6 μM Compound: 5	Antiproliferative activity against human BT549 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay Antiproliferative activity against human BT549 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 20804165]
DU-145	IC₅₀	2.7 μM Compound: 5	Antiproliferative activity against human DU145 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay Antiproliferative activity against human DU145 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 20804165]
HCC 2998	IC₅₀	2.9 μM Compound: 5	Antiproliferative activity against human HCC2998 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay Antiproliferative activity against human HCC2998 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 20804165]
HCT-116	IC₅₀	0.055 ug Compound: 6	Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting Cytotoxicity against human HCT116 cells measured per disk after 5 days by hemocytometer counting	[PMID: 21452840]
HT-29	IC₅₀	0.1 μM Compound: 7	Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay	[PMID: 23895019]
NCI-H460	IC₅₀	2 μM Compound: 5	Antiproliferative activity against human NCI-H460 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay Antiproliferative activity against human NCI-H460 cells after 96 hrs by CellTiter-Glo luminescent cell viability assay	[PMID: 20804165]
Raji	IC₅₀	2.4 μM Compound: 7	Cytotoxicity against human Raji cells after 72 hrs by MTS assay Cytotoxicity against human Raji cells after 72 hrs by MTS assay	[PMID: 23895019]
Raji	IC₅₀	5.6 μM Compound: 7	Cytotoxicity against human Raji cells after 48 hrs by MTS assay Cytotoxicity against human Raji cells after 48 hrs by MTS assay	[PMID: 23895019]
U-937	IC₅₀	0.07 μM Compound: 4, 12a-hydroxyrotenone	Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	0.09 μM Compound: 4, 12a-hydroxyrotenone	Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay	[PMID: 17158054]
U-937	IC₅₀	3.6 μM Compound: 4, 12a-hydroxyrotenone	Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay	[PMID: 17158054]

Molecular Weight

410.42

Formula

C₂₃H₂₂O₇

CAS No.

509-96-6

Appearance

Solid

Color

White to off-white

SMILES

O=C1[C@@]2([C@]([H])(OC3=C1C=CC4=C3C[C@@H](O4)C(C)=C)COC5=C2C=C(C(OC)=C5)OC)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (243.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4365 mL	12.1826 mL	24.3653 mL
5 mM	0.4873 mL	2.4365 mL	4.8731 mL
10 mM	0.2437 mL	1.2183 mL	2.4365 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (253 KB) HNMR (262 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Harinantenaina L, et, al. Antiproliferative compounds from Pongamiopsis pervilleana from the Madagascar Dry Forest. J Nat Prod. 2010 Sep 24;73(9):1559-62. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4365 mL	12.1826 mL	24.3653 mL	60.9132 mL
	5 mM	0.4873 mL	2.4365 mL	4.8731 mL	12.1826 mL
	10 mM	0.2437 mL	1.2183 mL	2.4365 mL	6.0913 mL
	15 mM	0.1624 mL	0.8122 mL	1.6244 mL	4.0609 mL
	20 mM	0.1218 mL	0.6091 mL	1.2183 mL	3.0457 mL
	25 mM	0.0975 mL	0.4873 mL	0.9746 mL	2.4365 mL
	30 mM	0.0812 mL	0.4061 mL	0.8122 mL	2.0304 mL
	40 mM	0.0609 mL	0.3046 mL	0.6091 mL	1.5228 mL
	50 mM	0.0487 mL	0.2437 mL	0.4873 mL	1.2183 mL
	60 mM	0.0406 mL	0.2030 mL	0.4061 mL	1.0152 mL
	80 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.7614 mL
	100 mM	0.0244 mL	0.1218 mL	0.2437 mL	0.6091 mL

Rotenolone Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rotenolone509-96-6Othersantiproliferativeovarian cancerbreast cancerprostate cancerNSCLCcolon cancerInhibitorinhibitorinhibit

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Rotenolone

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Complete Stock Solution Preparation Table

Rotenolone Related Classifications

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