(RS)-AMPA (Synonyms: (±)-AMPA)

Name: (RS)-AMPA
Brand: MedChemExpress
SKU: HY-100815B
Rating: 4.5 (1 reviews)

Cat. No.: HY-100815B Purity: ≥95.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

(RS)-AMPA ((±)-AMPA) is a glutamate analogue and a potent and selective excitatory neurotransmitter L-glutamic acid agonist. (RS)-AMPA does not interfere with binding sites for kainic acid or NMDA receptors.

For research use only. We do not sell to patients.

(RS)-AMPA Chemical Structure

CAS No. : 77521-29-0

Size	Price	Stock	Quantity
5 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 640	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1140	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2000	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

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AMPA Receptor NMDA Receptor Kainate Receptor

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	EC₅₀	16 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA4 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	37 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA3 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	53 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA1 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
HEK293	EC₅₀	67 μM Compound: (R,S)-AMPA	Agonist activity at rat recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay Agonist activity at rat recombinant GluA2 receptor flip isoform expressed in HEK293 cells assessed as effect on cyclothiazide-induced calcium flux by Fluo-4/AM staining-based fluorescence assay	[PMID: 20096591]
Oocyte	EC₅₀	1.4 μM Compound: 1 (AMPA)	Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 3 (GluR3) using rat cortical slice model in Xenopus laevis oocytes Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 3 (GluR3) using rat cortical slice model in Xenopus laevis oocytes	[PMID: 12747796]
Oocyte	EC₅₀	8.7 μM Compound: 1 (AMPA)	Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 1 using rat cortical slice model in Xenopus laevis oocytes Effective concentration of compound was determined for electrophysiological properties on Ionotropic glutamate receptor AMPA 1 using rat cortical slice model in Xenopus laevis oocytes	[PMID: 12747796]

In Vitro

(RS)-AMPA (10-3-10-4 M) causes depolarizations of cultured rat spinal and brainstem neurones. The depolarization by (RS)-AMPA is clearly dose-dependent, although there is a great variability of effects between individual neurones. Application of (RS)-AMPA at 10-5 M produces only small depolarizations (3-7 mV), whereas at 10-4 M, the amplitudes of the depolarizations ranged from 4 to 33 mV. (RS)-AMPA also causes an increase of the discharge rate of spontaneously firing neurones or sometimes evoked a short burst of action potentials in silent cells. (RS)-AMPA exerts its depolarizing effects by activating glutamate/quisqualate receptors without affecting NMDA receptors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

186.17

Formula

C₇H₁₀N₂O₄

CAS No.

77521-29-0

Appearance

Solid

Color

White to off-white

SMILES

O=C1C(CC(N)C(O)=O)=C(C)ON1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (537.14 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.3714 mL	26.8572 mL	53.7143 mL
5 mM	1.0743 mL	5.3714 mL	10.7429 mL
10 mM	0.5371 mL	2.6857 mL	5.3714 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥95.0%

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Data Sheet (274 KB) SDS (393 KB)

COA (251 KB) HNMR (231 KB)

Handling Instructions (2659 KB)

References

[1]. Hösli L, et al. Effects of the glutamate analogue AMPA and its interaction with antagonists on cultured rat spinal and brain stem neurones. Neurosci Lett. 1983 Mar 28;36(1):59-62. [Content Brief]

[2]. Sommer B, et al. Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS. Science. 1990 Sep 28;249(4976):1580-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.3714 mL	26.8572 mL	53.7143 mL	134.2859 mL
	5 mM	1.0743 mL	5.3714 mL	10.7429 mL	26.8572 mL
	10 mM	0.5371 mL	2.6857 mL	5.3714 mL	13.4286 mL
	15 mM	0.3581 mL	1.7905 mL	3.5810 mL	8.9524 mL
	20 mM	0.2686 mL	1.3429 mL	2.6857 mL	6.7143 mL
	25 mM	0.2149 mL	1.0743 mL	2.1486 mL	5.3714 mL
	30 mM	0.1790 mL	0.8952 mL	1.7905 mL	4.4762 mL
	40 mM	0.1343 mL	0.6714 mL	1.3429 mL	3.3571 mL
	50 mM	0.1074 mL	0.5371 mL	1.0743 mL	2.6857 mL
	60 mM	0.0895 mL	0.4476 mL	0.8952 mL	2.2381 mL
	80 mM	0.0671 mL	0.3357 mL	0.6714 mL	1.6786 mL
	100 mM	0.0537 mL	0.2686 mL	0.5371 mL	1.3429 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

(RS)-AMPA Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(RS)-AMPA77521-29-0(±)-AMPAiGluRIonotropic glutamate receptorsDepolarizationsmotoneuronesinterneuronesglutamateCNSkainicL-glutamicneurotransmitterNMDAInhibitorinhibitorinhibit

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(RS)-AMPA (Synonyms: (±)-AMPA)

Customer Review

Other Forms of (RS)-AMPA:

(RS)-AMPA Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All iGluR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

(RS)-AMPA Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

(RS)-AMPA Related Classifications

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