S-Trityl-L-cysteine (Synonyms: NSC 83265; S-Tritylcysteine; 3-Tritylthio-L-alanine)

Name: S-Trityl-L-cysteine
Brand: MedChemExpress
SKU: HY-W011102
Rating: 4.5 (1 reviews)

Cat. No.: HY-W011102 Purity: 99.10%: FAQs
Data Sheet SDS COA Handling Instructions

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S-Trityl-L-cysteine (NSC 83265) is a selective and allosteric kinesin Eg5 inhibitor with an IC₅₀ of 1 μM for the inhibition of basal ATPase activity and 140 nM for the microtubule-activated ATPase activity. S-Trityl-L-cysteine has antitumor activities.

For research use only. We do not sell to patients.

S-Trityl-L-cysteine Chemical Structure

CAS No. : 2799-07-7

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
50 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

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View All Isoforms

Kinesin-5/Eg5/KSP Kinesin-6 Kinesin-7/CENP-E Kinesin-12 Kinesin-14

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Eg5

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	GI₅₀	1563 nM Compound: 4	Growth inhibition of human BxPC3 cells after 72 hrs by Alamar blue assay Growth inhibition of human BxPC3 cells after 72 hrs by Alamar blue assay	[PMID: 23394180]
BXPC-3	GI₅₀	1563 nM Compound: 4, STLC	Cytotoxicity against human BxPC3 cells after 72 hrs by Alamar blue assay Cytotoxicity against human BxPC3 cells after 72 hrs by Alamar blue assay	[PMID: 22749640]
BXPC-3	GI₅₀	1563 nM Compound: 1, STLC	Antiproliferative activity against human BxPC3 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human BxPC3 cells after 72 hrs by Alamar blue assay	[PMID: 22248262]
HCT-116	GI₅₀	553 nM Compound: 4	Growth inhibition of human HCT116 cells after 72 hrs by Alamar blue assay Growth inhibition of human HCT116 cells after 72 hrs by Alamar blue assay	[PMID: 23394180]
HCT-116	GI₅₀	553 nM Compound: 4, STLC	Cytotoxicity against human HCT116 cells after 72 hrs by Alamar blue assay Cytotoxicity against human HCT116 cells after 72 hrs by Alamar blue assay	[PMID: 22749640]
HCT-116	GI₅₀	553 nM Compound: 1, STLC	Antiproliferative activity against human HCT116 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human HCT116 cells after 72 hrs by Alamar blue assay	[PMID: 22248262]
HCT-116	IC₅₀	910 nM Compound: 3; STLC	Growth inhibition of human HCT116 cells after 72 hrs by MTS assay Growth inhibition of human HCT116 cells after 72 hrs by MTS assay	[PMID: 26396688]
HeLa	EC₅₀	0.7 μM Compound: 1, STLC	Induction of mitotic arrest in human HeLa cells assessed as monoastral spindle formation after 24 hrs Induction of mitotic arrest in human HeLa cells assessed as monoastral spindle formation after 24 hrs	[PMID: 18266314]
HeLa	EC₅₀	1.7 μM Compound: 1, STLC	Induction of mitotic arrest in human HeLa cells after 24 hrs Induction of mitotic arrest in human HeLa cells after 24 hrs	[PMID: 18266314]
K562	GI₅₀	1452 nM Compound: 4, STLC	Cytotoxicity against human K562 cells after 72 hrs by Alamar blue assay Cytotoxicity against human K562 cells after 72 hrs by Alamar blue assay	[PMID: 22749640]
K562	GI₅₀	1452 nM Compound: 1, STLC	Antiproliferative activity against human K562 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human K562 cells after 72 hrs by Alamar blue assay	[PMID: 22248262]
K562	GI₅₀	1791 nM Compound: 4	Growth inhibition of human K562 cells after 72 hrs by Alamar blue assay Growth inhibition of human K562 cells after 72 hrs by Alamar blue assay	[PMID: 23394180]
LNCaP	GI₅₀	811 nM Compound: 4	Growth inhibition of human LNCAP cells after 72 hrs by Alamar blue assay Growth inhibition of human LNCAP cells after 72 hrs by Alamar blue assay	[PMID: 23394180]
NCI-H1299	GI₅₀	1549 nM Compound: 4	Growth inhibition of human NCI-H1299 cells after 72 hrs by Alamar blue assay Growth inhibition of human NCI-H1299 cells after 72 hrs by Alamar blue assay	[PMID: 23394180]
NCI-H1299	GI₅₀	1549 nM Compound: 4, STLC	Cytotoxicity against human NCI-H1299 cells after 72 hrs by Alamar blue assay Cytotoxicity against human NCI-H1299 cells after 72 hrs by Alamar blue assay	[PMID: 22749640]
NCI-H1299	GI₅₀	1549 nM Compound: 1, STLC	Antiproliferative activity against human NCI-H1299 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human NCI-H1299 cells after 72 hrs by Alamar blue assay	[PMID: 22248262]
PC-3	GI₅₀	1371 nM Compound: 4	Growth inhibition of human PC3 cells after 72 hrs by Alamar blue assay Growth inhibition of human PC3 cells after 72 hrs by Alamar blue assay	[PMID: 23394180]

In Vitro

S-Trityl-L-cysteine binds to a unique pocket in the Eg5 motor domain formed by secondary structural elements (helix a2/loop L5/helix a3)^[1].
S-Trityl-L-cysteine (1-20 μM; for 72 h) could mediate cell apoptosis, as well as cell cycle arrest, in a dose-dependent manner. S-Trityl-L-cysteine-mediated apoptosis and cell cycle arrest were triggered by activation of the mitogen-activated protein kinase and nuclear factor kB signaling pathways^[1].
S-Trityl-L-cysteine induces mitotic arrest in HeLa cells (IC50 of 700 nM) with characteristic monoastral spindles^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

363.47

Formula

C₂₂H₂₁NO₂S

CAS No.

2799-07-7

Appearance

Solid

Color

White to off-white

SMILES

N[C@@H](CSC(C1=CC=CC=C1)(C2=CC=CC=C2)C3=CC=CC=C3)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (68.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7513 mL	13.7563 mL	27.5126 mL
5 mM	0.5503 mL	2.7513 mL	5.5025 mL
10 mM	0.2751 mL	1.3756 mL	2.7513 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (2.75 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.10%

Select Batch:

Data Sheet (278 KB) SDS (392 KB)

COA (255 KB) HNMR (123 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

References

[1]. Wei Wu, et al. S-trityl-L-cysteine, a novel Eg5 inhibitor, is a potent chemotherapeutic strategy in neuroblastoma. Oncol Lett. 2018 Jul;16(1):1023-1030. [Content Brief]

[2]. Salvatore DeBonis, et al. In vitro screening for inhibitors of the human mitotic kinesin Eg5 with antimitotic and antitumor activities. Mol Cancer Ther. 2004 Sep;3(9):1079-90. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7513 mL	13.7563 mL	27.5126 mL	68.7815 mL
	5 mM	0.5503 mL	2.7513 mL	5.5025 mL	13.7563 mL
	10 mM	0.2751 mL	1.3756 mL	2.7513 mL	6.8781 mL
	15 mM	0.1834 mL	0.9171 mL	1.8342 mL	4.5854 mL
	20 mM	0.1376 mL	0.6878 mL	1.3756 mL	3.4391 mL
	25 mM	0.1101 mL	0.5503 mL	1.1005 mL	2.7513 mL
	30 mM	0.0917 mL	0.4585 mL	0.9171 mL	2.2927 mL
	40 mM	0.0688 mL	0.3439 mL	0.6878 mL	1.7195 mL
	50 mM	0.0550 mL	0.2751 mL	0.5503 mL	1.3756 mL
	60 mM	0.0459 mL	0.2293 mL	0.4585 mL	1.1464 mL

S-Trityl-L-cysteine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

S-Trityl-L-cysteine2799-07-7NSC 83265 S-Tritylcysteine 3-Tritylthio-L-alanineNSC83265NSC-83265KinesinApoptosiskinesin Eg5ATPase activityantitumor activitiescell cycle arrestInhibitorinhibitorinhibit

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S-Trityl-L-cysteine (Synonyms: NSC 83265; S-Tritylcysteine; 3-Tritylthio-L-alanine)

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S-Trityl-L-cysteine Related Antibodies

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Kinesin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

S-Trityl-L-cysteine Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

S-Trityl-L-cysteine Related Classifications

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