2. Membrane Transporter/Ion Channel
  3. Sodium Channel
  4. S3969

S3969 is a potent and reversible activator of the human epithelial sodium channel (hENaC). The apparent EC50 for S3969 activation of hENaC is 1.2 μm.

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S3969 Chemical Structure

S3969 Chemical Structure

CAS No. : 1027997-01-8

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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

S3969 is a potent and reversible activator of the human epithelial sodium channel (hENaC). The apparent EC50 for S3969 activation of hENaC is 1.2 μm[1].

In Vitro

S3969 fully and reversibly activates hENaC in heterologous cells. S3969 activates δβγ, αβγ, α2βγ, δ2βγ, and αβG37Sγ hENaC with EC50s of 1.2±0.2 μM, 1.2±0.1 μM, 1.2±0.5, 0.4±0.1,and 1.2±0.4 μM[1].
S3969 does not activate αβγ mouse ENaC (mENaC) at concentrations yielding maximal activation of αβγ hENaC or δβγ hENaC. Weak αβγ mENaC activation was observed at high concentrations of S3969 (100-300 μm)[1].
S3969 exhibits high efficacy (600-700% hENaC activation at 30 μm) as well as potency on wild-type hENaC (apparent EC50 ∼1 μm for αβγ hENaC and δβγ hENaC but not αβγ mENaC) [1].
ENaC-activator S3969 (10 µM) stimulates αF61L mutant ENaC more than wild-type ENaC[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

S3969 Related Antibodies
Molecular Weight

320.45

Formula

C17H24N2O2S
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

CC(C)CC(SC1=CNC2=C1C(C)=CC=C2)C(NCCO)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (312.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1206 mL 15.6031 mL 31.2061 mL
5 mM 0.6241 mL 3.1206 mL 6.2412 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (7.80 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1206 mL 15.6031 mL 31.2061 mL 78.0153 mL
5 mM 0.6241 mL 3.1206 mL 6.2412 mL 15.6031 mL
10 mM 0.3121 mL 1.5603 mL 3.1206 mL 7.8015 mL
15 mM 0.2080 mL 1.0402 mL 2.0804 mL 5.2010 mL
20 mM 0.1560 mL 0.7802 mL 1.5603 mL 3.9008 mL
25 mM 0.1248 mL 0.6241 mL 1.2482 mL 3.1206 mL
30 mM 0.1040 mL 0.5201 mL 1.0402 mL 2.6005 mL
40 mM 0.0780 mL 0.3901 mL 0.7802 mL 1.9504 mL
50 mM 0.0624 mL 0.3121 mL 0.6241 mL 1.5603 mL
60 mM 0.0520 mL 0.2601 mL 0.5201 mL 1.3003 mL
80 mM 0.0390 mL 0.1950 mL 0.3901 mL 0.9752 mL
100 mM 0.0312 mL 0.1560 mL 0.3121 mL 0.7802 mL
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S3969 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

S39691027997-01-8S 3969S-3969Sodium ChannelNa channelsNa+ channelsreversible activatorhuman epithelial sodium channelhENaCInhibitorinhibitorinhibit

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