Sinefungin (Synonyms: Adenosyl-Ornithine; A-9145; Antibiotic 32232RP)

Name: Sinefungin
Brand: MedChemExpress
SKU: HY-101938
Rating: 5.0 (3 reviews)

Cat. No.: HY-101938 Purity: 99.91%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Sinefungin is a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase, and viral multiplication. Sinefungin, a SET7/9 inhibitor, ameliorates renal fibrosis by inhibiting H3K4 methylation.

For research use only. We do not sell to patients.

Sinefungin Chemical Structure

CAS No. : 58944-73-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 319	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 319	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 270	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 600	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1280	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Sinefungin

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Sinefungin is a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase, and viral multiplication^[1]. Sinefungin, a SET7/9 inhibitor, ameliorates renal fibrosis by inhibiting H3K4 methylation^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	CC₅₀	72.93 μM Compound: Sinefungin	Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by neutral red uptake assay Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by neutral red uptake assay	[PMID: 34257831]
HEK293	CC₅₀	> 500 μM Compound: SFG	Cytotoxicity against human HEK293 cells assessed as cell viability at 100 uM measured after 24 hrs by MTT assay Cytotoxicity against human HEK293 cells assessed as cell viability at 100 uM measured after 24 hrs by MTT assay	[PMID: 35849534]
HepG2	CC₅₀	> 100 μM Compound: Sinefungin	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by neutral red uptake assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 48 hrs by neutral red uptake assay	[PMID: 34257831]
Sf9	IC₅₀	8.6 μM Compound: 1	Inhibition of full-length N-terminal FLAG-tagged PRMT5 (unknown origin) (1 to 637 residues) expressed in baculovirus infected Sf9 insect cells using histone H4 as peptide after 5 hrs in presence of [H3]AdoMet by scintillation proximity assay Inhibition of full-length N-terminal FLAG-tagged PRMT5 (unknown origin) (1 to 637 residues) expressed in baculovirus infected Sf9 insect cells using histone H4 as peptide after 5 hrs in presence of [H3]AdoMet by scintillation proximity assay	[PMID: 30366617]

In Vitro

Sinefungin (0.5 or 1.0 μg/mL, 60 minutes) ameliorates the TGF-β1-induced increase of α-SMA and inhibits the upregulation of histone H3K4 monomethylation in renal epithelial cells and renal fibroblast cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	Renal epithelial cells.
Concentration:	0.5 or 1.0 μg/mL.
Incubation Time:	Pretreatment 60 minutes before TGF-β1 (10 ng/mL).
Result:	Significantly reduced TGF-β1-inducedα-SMA protein expression and inhibited H3K4me1 in a dose-dependent manner in both NRK-52E and NRK-49F cells.

In Vivo

Sinefungin (10 mg/kg, per day immediately after UUO) ameliorates renal fibrosis in obstructive nephropathy^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6J mice (8 weeks of age)^[2].
Dosage:	10 mg/kg
Administration:	Administered intraperitoneally per day immediately after UUO (prepared as a suspension in distilled water and 0.9% NaCl solution).
Result:	Inhibited α-SMA protein expression. Ameliorated those (α-SMA, FSP-1, collagen 1, collagen 3) both at 3 and 7 days after UUO.

Molecular Weight

381.39

Formula

C₁₅H₂₃N₇O₅

CAS No.

58944-73-3

Appearance

Solid

Color

White to yellow

SMILES

O[C@H]([C@@H]1O)[C@@H](O[C@@H]1C[C@@H](N)CC[C@H](N)C(O)=O)N2C(N=CN=C3N)=C3N=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (262.20 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6220 mL	13.1099 mL	26.2199 mL
5 mM	0.5244 mL	2.6220 mL	5.2440 mL
10 mM	0.2622 mL	1.3110 mL	2.6220 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.91%

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Data Sheet (274 KB) SDS (392 KB)

COA (247 KB) HNMR (246 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Pugh CS, et al. Sinefungin, a potent inhibitor of virion mRNA(guanine-7-)-methyltransferase, mRNA(nucleoside-2'-)-methyltransferase, and viral multiplication. J Biol Chem. 1978 Jun 25;253(12):4075-7. [Content Brief]

[2]. Sasaki K, et al. Inhibition of SET Domain-Containing Lysine Methyltransferase 7/9 Ameliorates Renal Fibrosis. J Am Soc Nephrol. 2016 Jan;27(1):203-15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.6220 mL	13.1099 mL	26.2199 mL	65.5497 mL
	5 mM	0.5244 mL	2.6220 mL	5.2440 mL	13.1099 mL
	10 mM	0.2622 mL	1.3110 mL	2.6220 mL	6.5550 mL
	15 mM	0.1748 mL	0.8740 mL	1.7480 mL	4.3700 mL
	20 mM	0.1311 mL	0.6555 mL	1.3110 mL	3.2775 mL
	25 mM	0.1049 mL	0.5244 mL	1.0488 mL	2.6220 mL
	30 mM	0.0874 mL	0.4370 mL	0.8740 mL	2.1850 mL
	40 mM	0.0655 mL	0.3277 mL	0.6555 mL	1.6387 mL
	50 mM	0.0524 mL	0.2622 mL	0.5244 mL	1.3110 mL
	60 mM	0.0437 mL	0.2185 mL	0.4370 mL	1.0925 mL
	80 mM	0.0328 mL	0.1639 mL	0.3277 mL	0.8194 mL
	100 mM	0.0262 mL	0.1311 mL	0.2622 mL	0.6555 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Sinefungin Related Classifications

Inflammation/Immunology Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Sinefungin (Synonyms: Adenosyl-Ornithine; A-9145; Antibiotic 32232RP)

Customer Review

Sinefungin Related Antibodies

3 Publications Citing Use of MCE Sinefungin

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Histone Methyltransferase Isoform Specific Products:

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View All Parasite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sinefungin Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sinefungin Related Classifications

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