SIRT1-IN-2

Name: SIRT1-IN-2
Brand: MedChemExpress
SKU: HY-146689
Rating: 4.5 (1 reviews)

Cat. No.: HY-146689 Purity: 98.86%: FAQs
Data Sheet SDS COA Handling Instructions

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SIRT1-IN-2 (compound 3h) is a potent and selective SIRT1 (silent information regulator 1) inhibitor, with an IC₅₀ of 1.6 μM.

For research use only. We do not sell to patients.

SIRT1-IN-2 Chemical Structure

CAS No. : 2470969-89-0

Size	Price	Stock	Quantity
5 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 480	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2480	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Sirtuin SIRT1 SIRT2 SIRT3 SIRT6 SIRT5 SIRT7

Biological Activity
Purity & Documentation
References
Customer Review

Description

SIRT1-IN-2 (compound 3h) is a potent and selective SIRT1 (silent information regulator 1) inhibitor, with an IC₅₀ of 1.6 μM^[1].

IC₅₀ & Target

SIRT1

1.6 μM (IC₅₀)

SIRT2

39 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	48 μM Compound: 3h	Cytotoxicity against human A549 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
HCT-116	IC₅₀	37 μM Compound: 3h	Cytotoxicity against human HCT-116 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HCT-116 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
HEK-293T	IC₅₀	> 100 μM Compound: 3h	Cytotoxicity against human HEK293T cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HEK293T cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
HepG2	IC₅₀	40 μM Compound: 3h	Cytotoxicity against human HepG2 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
HT-29	IC₅₀	> 100 μM Compound: 3h	Cytotoxicity against human HT-29 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
HUVEC	IC₅₀	45 μM Compound: 3h	Cytotoxicity against human HUVEC cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human HUVEC cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
K562	IC₅₀	51 μM Compound: 3h	Cytotoxicity against human K562 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human K562 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
MCF7	IC₅₀	48 μM Compound: 3h	Cytotoxicity against human MCF7 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]
NCI-H460	IC₅₀	> 100 μM Compound: 3h	Cytotoxicity against human NCI-H460 cells assessed as cell viability measured after 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as cell viability measured after 48 hrs by MTT assay	[PMID: 32711231]

In Vitro

SIRT1-IN-2 (compound 3h) (0-100 μM, 48 h) inhibits the proliferation of Human cancer cell lines including K562, HCT-116, HepG2, A549, and MCF-7, and shows significantly less cytotoxic on 293T cells and HUVEC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:	Human cancer cell lines (K562, HCT-116, H460, HepG2, A549, HT-29, MCF-7) and normal cell lines (293T, HUVEC). ^[1]
Concentration:	0, 0.01, 0.1, 1, 10, 100 μM
Incubation Time:	48 h
Result:	Inhibited the proliferation of Human cancer cell lines including K562, HCT-116, HepG2, A549, and MCF-7, with IC₅₀ values of 51, 37, 40, 48, and 48 μM, respectively. And showed significantly less cytotoxic on 293T cells and HUVEC, with IC₅₀ values of > 100 and 45 μM, respectively.

Molecular Weight

250.72

Formula

C₁₃H₁₅ClN₂O

CAS No.

2470969-89-0

Appearance

Solid

Color

White to light yellow

SMILES

CC(C1=C(NC2=CC=C(C=C21)Cl)CC(N)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (398.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9885 mL	19.9426 mL	39.8851 mL
5 mM	0.7977 mL	3.9885 mL	7.9770 mL
10 mM	0.3989 mL	1.9943 mL	3.9885 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.86%

Select Batch:

Data Sheet (272 KB) SDS (251 KB)

COA (248 KB) HNMR (266 KB) RP-HPLC (263 KB) MS (75 KB)

Handling Instructions (2659 KB)

References

[1]. Laaroussi H, Ding Y, Teng Y, et al. Synthesis of indole inhibitors of silent information regulator 1 (SIRT1), and their evaluation as cytotoxic agents. Eur J Med Chem. 2020;202:112561. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9885 mL	19.9426 mL	39.8851 mL	99.7128 mL
	5 mM	0.7977 mL	3.9885 mL	7.9770 mL	19.9426 mL
	10 mM	0.3989 mL	1.9943 mL	3.9885 mL	9.9713 mL
	15 mM	0.2659 mL	1.3295 mL	2.6590 mL	6.6475 mL
	20 mM	0.1994 mL	0.9971 mL	1.9943 mL	4.9856 mL
	25 mM	0.1595 mL	0.7977 mL	1.5954 mL	3.9885 mL
	30 mM	0.1330 mL	0.6648 mL	1.3295 mL	3.3238 mL
	40 mM	0.0997 mL	0.4986 mL	0.9971 mL	2.4928 mL
	50 mM	0.0798 mL	0.3989 mL	0.7977 mL	1.9943 mL
	60 mM	0.0665 mL	0.3324 mL	0.6648 mL	1.6619 mL
	80 mM	0.0499 mL	0.2493 mL	0.4986 mL	1.2464 mL
	100 mM	0.0399 mL	0.1994 mL	0.3989 mL	0.9971 mL

SIRT1-IN-2 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SIRT1-IN-22470969-89-0SirtuinIndolesCancerCytotoxicityInhibitorinhibitorinhibit

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SIRT1-IN-2

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SIRT1-IN-2 Related Antibodies

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Biological Activity

Purity & Documentation

References

Customer Review

SIRT1-IN-2 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

SIRT1-IN-2 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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