2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. SM-324405

SM-324405 is a TLR7 agonistic antedrug (EC50 = 50 nM), with pEC50 values of 7.3 and 6.6 for human TLR7 and Rat TLR7, respectively. SM-324405 is used for immunoresearch of allergic diseases. An antedrug is defined as a locally active compound that is designed to be rapidly metabolized to an inactive form upon entry into the circulation and prevents systemic toxicity by losing its agonistic activity in a plasmatic environment.

For research use only. We do not sell to patients.

SM-324405 Chemical Structure

SM-324405 Chemical Structure

CAS No. : 677773-91-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 160 In-stock
Solution
10 mM * 1 mL in DMSO USD 160 In-stock
Solid
1 mg USD 46 In-stock
5 mg USD 145 In-stock
10 mg USD 230 In-stock
25 mg USD 430 In-stock
50 mg USD 680 In-stock
100 mg USD 1050 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

SM-324405 Related Antibodies

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Description

SM-324405 is a TLR7 agonistic antedrug (EC50 = 50 nM), with pEC50 values of 7.3 and 6.6 for human TLR7 and Rat TLR7, respectively. SM-324405 is used for immunoresearch of allergic diseases. An antedrug is defined as a locally active compound that is designed to be rapidly metabolized to an inactive form upon entry into the circulation and prevents systemic toxicity by losing its agonistic activity in a plasmatic environment[1][2][3].

IC50 & Target[1]

TLR7

50 nM (EC50)

human TLR7

7.3 (pEC50)

Rat TLR7

6.6 (pEC50)

In Vitro

SM-324405 is a novel candidate for immunotherapy of allergic diseases[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SM-324405 Related Antibodies
In Vivo

SM-324405 (9e, intratracheal administration) effectively inhibits allergen-induced airway inflammation without inducing cytokines systemically[1].
SM-324405 is metabolized to the corresponding acid in human plasma with t1/2 of 2.6 min[1][2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

385.42

Formula

C19H23N5O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OC)CC1=CC=CC(CN2C(NC3=C(N)N=C(OCCCC)N=C23)=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (129.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5946 mL 12.9729 mL 25.9457 mL
5 mM 0.5189 mL 2.5946 mL 5.1891 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5946 mL 12.9729 mL 25.9457 mL 64.8643 mL
5 mM 0.5189 mL 2.5946 mL 5.1891 mL 12.9729 mL
10 mM 0.2595 mL 1.2973 mL 2.5946 mL 6.4864 mL
15 mM 0.1730 mL 0.8649 mL 1.7297 mL 4.3243 mL
20 mM 0.1297 mL 0.6486 mL 1.2973 mL 3.2432 mL
25 mM 0.1038 mL 0.5189 mL 1.0378 mL 2.5946 mL
30 mM 0.0865 mL 0.4324 mL 0.8649 mL 2.1621 mL
40 mM 0.0649 mL 0.3243 mL 0.6486 mL 1.6216 mL
50 mM 0.0519 mL 0.2595 mL 0.5189 mL 1.2973 mL
60 mM 0.0432 mL 0.2162 mL 0.4324 mL 1.0811 mL
80 mM 0.0324 mL 0.1622 mL 0.3243 mL 0.8108 mL
100 mM 0.0259 mL 0.1297 mL 0.2595 mL 0.6486 mL
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SM-324405 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SM-324405677773-91-0SM324405SM 324405Toll-like Receptor (TLR)allergicdiseasesimmunotherapycytokineTLR7low toxicityInhibitorinhibitorinhibit

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