2. Epigenetics
  3. HuR
  4. SRI-42127

SRI-42127 is a HuR translocation inhibitor. HuR is an RNA regulator that binds to AREs, and HuR translocations promote the production of inflammatory cytokines in glial cells. However, SRI-42127 can destroy mRNA stability and inhibit gene promoter activation. SRI-42127 also inhibits microglial cell activation and attenuates recruitment/chemotaxis of neutrophils and monocytes.

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SRI-42127 Chemical Structure

SRI-42127 Chemical Structure

CAS No. : 2727872-68-4

Size Price Stock Quantity
5 mg USD 170 In-stock
10 mg USD 310 In-stock
25 mg USD 620 In-stock
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Based on 1 publication(s) in Google Scholar

SRI-42127 Related Antibodies

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Description

SRI-42127 is a HuR translocation inhibitor. HuR is an RNA regulator that binds to AREs, and HuR translocations promote the production of inflammatory cytokines in glial cells. However, SRI-42127 can destroy mRNA stability and inhibit gene promoter activation. SRI-42127 also inhibits microglial cell activation and attenuates recruitment/chemotaxis of neutrophils and monocytes[1].

IC50 & Target

HuR[1]
In Vitro

SRI-42127 (0.05-1 μM; 24 h) blocks HuR cytoplasmic translocation stimulated by LPS (1 μg/mL) in primary microglia[1].
SRI-42127 (0.05-1 μM; 24 h) suppresses inflammatory cytokine and chemokine mRNA in activated Primary microglia cells (PMG)[1].
SRI-42127 (0.5 μM and 1 μM; 24 h) blocks glial-produced chemoattraction/migration signals for neutrophils and monocytes[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

SRI-42127 Related Antibodies
In Vivo

SRI-42127 (15 mg/kg; i.p.; single dose) suppresses HuR translocation and the activation of microglia in mice model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

348.40

Formula

C19H20N6O
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CN(C1CCOCC1)C2=NN3C(C=C2)=NC=C3C4=CC5=C(C=C4)C=NN5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (143.51 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8703 mL 14.3513 mL 28.7026 mL
5 mM 0.5741 mL 2.8703 mL 5.7405 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 12.5 mg/mL (35.88 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.80%

COA (251 KB) HNMR (144 KB) RP-HPLC (168 KB) LCMS (386 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8703 mL 14.3513 mL 28.7026 mL 71.7566 mL
5 mM 0.5741 mL 2.8703 mL 5.7405 mL 14.3513 mL
10 mM 0.2870 mL 1.4351 mL 2.8703 mL 7.1757 mL
15 mM 0.1914 mL 0.9568 mL 1.9135 mL 4.7838 mL
20 mM 0.1435 mL 0.7176 mL 1.4351 mL 3.5878 mL
25 mM 0.1148 mL 0.5741 mL 1.1481 mL 2.8703 mL
30 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3919 mL
40 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7939 mL
50 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4351 mL
60 mM 0.0478 mL 0.2392 mL 0.4784 mL 1.1959 mL
80 mM 0.0359 mL 0.1794 mL 0.3588 mL 0.8970 mL
100 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7176 mL
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SRI-42127 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SRI-421272727872-68-4SRI42127SRI 42127HuRHuman Antigen RInhibitorinhibitorinhibit

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