Steviol

Name: Steviol
Brand: MedChemExpress
SKU: HY-N2057
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2057 Purity: 99.69%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Steviol is a major metabolite of the sweetening compound stevioside. Steviol slows renal cyst growth by reducing AQP2 expression and promoting AQP2 degradation.

For research use only. We do not sell to patients.

Steviol Chemical Structure

CAS No. : 471-80-7

Size	Price	Stock	Quantity
1 mg	USD 56	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 119	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 202	In-stock	Estimated Time of Arrival: December 31
20 mg	USD 343	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Steviol is a major metabolite of the sweetening compound stevioside. Steviol slows renal cyst growth by reducing AQP2 expression and promoting AQP2 degradation^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity human against A549 cells measured after 48 hrs by CCK8 assay Antiproliferative activity human against A549 cells measured after 48 hrs by CCK8 assay	[PMID: 35943432]
HCT-116	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against human HCT-116 cells measured after 48 hrs by CCK8 assay Antiproliferative activity against human HCT-116 cells measured after 48 hrs by CCK8 assay	[PMID: 35943432]
HeLa	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against human HeLa cells measured after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells measured after 48 hrs by CCK8 assay	[PMID: 35943432]
Huh-7	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against human Huh-7 cells measured after 48 hrs by CCK8 assay Antiproliferative activity against human Huh-7 cells measured after 48 hrs by CCK8 assay	[PMID: 35943432]
MCF7	IC₅₀	185 μM Compound: 2	Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay	[PMID: 30059803]
MCF7	IC₅₀	185 μM Compound: 1	Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by inverted-phase contrast microscopy Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by inverted-phase contrast microscopy	[PMID: 35943432]
MDA-MB-231	IC₅₀	> 10 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by CCK8 assay Antiproliferative activity against human MDA-MB-231 cells measured after 48 hrs by CCK8 assay	[PMID: 35943432]
Raji	IC₅₀	340 μM Compound: 6	Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique Inhibition of TPA-induced EBV-early antigen activation in human Raji cells after 48hrs by indirect immunofluorescence technique	[PMID: 19131254]

Clinical Trial

Molecular Weight

318.45

Formula

C₂₀H₃₀O₃

CAS No.

471-80-7

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(C(O)=O)CCC[C@@]2(C)[C@@]3([H])[C@@]4(CC([C@@](CC3)(O)C4)=C)CC[C@]12[H]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (314.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1402 mL	15.7011 mL	31.4021 mL
5 mM	0.6280 mL	3.1402 mL	6.2804 mL
10 mM	0.3140 mL	1.5701 mL	3.1402 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.85 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.69%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (259 KB) HNMR (282 KB) LCMS (255 KB)

Handling Instructions (2659 KB)

References

[1]. Noitem R, et al. Steviol slows renal cyst growth by reducing AQP2 expression and promoting AQP2 degradation. Biomed Pharmacother. 2018 May;101:754-762. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1402 mL	15.7011 mL	31.4021 mL	78.5053 mL
	5 mM	0.6280 mL	3.1402 mL	6.2804 mL	15.7011 mL
	10 mM	0.3140 mL	1.5701 mL	3.1402 mL	7.8505 mL
	15 mM	0.2093 mL	1.0467 mL	2.0935 mL	5.2337 mL
	20 mM	0.1570 mL	0.7851 mL	1.5701 mL	3.9253 mL
	25 mM	0.1256 mL	0.6280 mL	1.2561 mL	3.1402 mL
	30 mM	0.1047 mL	0.5234 mL	1.0467 mL	2.6168 mL
	40 mM	0.0785 mL	0.3925 mL	0.7851 mL	1.9626 mL
	50 mM	0.0628 mL	0.3140 mL	0.6280 mL	1.5701 mL
	60 mM	0.0523 mL	0.2617 mL	0.5234 mL	1.3084 mL
	80 mM	0.0393 mL	0.1963 mL	0.3925 mL	0.9813 mL
	100 mM	0.0314 mL	0.1570 mL	0.3140 mL	0.7851 mL

Steviol Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Steviol471-80-7AquaporinAQPCFTRPolycystickidneydiseasePKDInhibitorinhibitorinhibit

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Steviol

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Other Forms of Steviol:

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Complete Stock Solution Preparation Table

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