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  4. Sulfo-CY-5.5 NHS ester tripotassium

Sulfo-CY-5.5 NHS ester tripotassium  (Synonyms: Sulfo-Cyanine5.5 NHS ester tripotassium)

Cat. No.: HY-D1389 Purity: 96.64%
COA Handling Instructions

Molarity Calculator

Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging.

For research use only. We do not sell to patients.

Sulfo-CY-5.5 NHS ester tripotassium Chemical Structure

Sulfo-CY-5.5 NHS ester tripotassium Chemical Structure

CAS No. : 2419286-92-1

Size Price Stock Quantity
5 mg USD 550 In-stock
10 mg USD 850 In-stock
25 mg USD 1700 In-stock
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Sulfo-CY-5.5 NHS ester tripotassium Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging[1].
Molecular Weight

1114.37

Formula

C44H42K3N3O16S4
CAS No.
Appearance

Solid

Color

Blue to dark blue

SMILES

O=C(CCCCC[N+]1=C(/C=C/C=C/C=C2N(C)C3=CC=C4C(C=C(S(=O)(O[K])=O)C=C4S(=O)(O[K])=O)=C3C/2(C)C)C(C)(C)C5=C1C=CC6=C5C=C(S(=O)(O[K])=O)C=C6S(=O)([O-])=O)ON7C(CCC7=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (44.87 mM)

DMSO : 25 mg/mL (22.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8974 mL 4.4868 mL 8.9737 mL
5 mM 0.1795 mL 0.8974 mL 1.7947 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (1.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (1.12 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 0.8974 mL 4.4868 mL 8.9737 mL 22.4342 mL
5 mM 0.1795 mL 0.8974 mL 1.7947 mL 4.4868 mL
10 mM 0.0897 mL 0.4487 mL 0.8974 mL 2.2434 mL
15 mM 0.0598 mL 0.2991 mL 0.5982 mL 1.4956 mL
20 mM 0.0449 mL 0.2243 mL 0.4487 mL 1.1217 mL
H2O 25 mM 0.0359 mL 0.1795 mL 0.3589 mL 0.8974 mL
30 mM 0.0299 mL 0.1496 mL 0.2991 mL 0.7478 mL
40 mM 0.0224 mL 0.1122 mL 0.2243 mL 0.5609 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Sulfo-CY-5.5 NHS ester tripotassium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfo-CY-5.5 NHS ester tripotassium2419286-92-1Sulfo-Cyanine5.5 NHS ester tripotassiumFluorescent DyeCyanine5.5antibodiesproteinsNIR imagingInhibitorinhibitorinhibit

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