Sulfo-Cy7.5 NHS ester

Cat. No.: HY-D1568: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Sulfo-Cy7.5 NHS ester is a near infrared water soluble hydrophilic dye, also is an NHS ester for the modification of amine groups. Sulfo-Cy7.5 NHS ester contains a trimeth ylene bridge and has a linker arm for its attachment to proteins, peptides, and other molecules. Sulfo-Cy7.5 NHS ester can be used for the research of NIR imaging applications.

For research use only. We do not sell to patients.

Sulfo-Cy7.5 NHS ester Chemical Structure

CAS No. : 2736437-44-6

Size	Price	Stock
1 mg	USD 110	Ask For Quote & Lead Time
5 mg	USD 375	Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

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Biological Activity
Purity & Documentation
References
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Description

Molecular Weight

1104.29

Formula

C₄₉H₅₀KN₃O₁₆S₄

CAS No.

2736437-44-6

Appearance

Solid

Color

Light green to green

SMILES

CN(/C(C1(C)C)=C/C=C2CCCC(/C=C/C3=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C(C=CC5=C6C=C(S(=O)(O[K])=O)C=C5S([O-])(=O)=O)=C6C3(C)C)=C\2)C7=C1C(C=C(S(O)(=O)=O)C=C8S(O)(=O)=O)=C8C=C7

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (45.28 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9056 mL	4.5278 mL	9.0556 mL
5 mM	0.1811 mL	0.9056 mL	1.8111 mL
10 mM	0.0906 mL	0.4528 mL	0.9056 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (1.13 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (1.13 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥96.0%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (262 KB)

Handling Instructions (2659 KB)

References

[1]. Pavel N Melentiev, et al. Ultrafast, Ultrasensitive Detection and Imaging of Single Cardiac Troponin-T Molecules. ACS Sens. 2020 Nov 25;5(11):3576-3583. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9056 mL	4.5278 mL	9.0556 mL	22.6390 mL
	5 mM	0.1811 mL	0.9056 mL	1.8111 mL	4.5278 mL
	10 mM	0.0906 mL	0.4528 mL	0.9056 mL	2.2639 mL
	15 mM	0.0604 mL	0.3019 mL	0.6037 mL	1.5093 mL
	20 mM	0.0453 mL	0.2264 mL	0.4528 mL	1.1319 mL
	25 mM	0.0362 mL	0.1811 mL	0.3622 mL	0.9056 mL
	30 mM	0.0302 mL	0.1509 mL	0.3019 mL	0.7546 mL
	40 mM	0.0226 mL	0.1132 mL	0.2264 mL	0.5660 mL