2. MAPK/ERK Pathway Stem Cell/Wnt Immunology/Inflammation
  3. MEK ERK AP-1
  4. TERT activator-1

TERT activator-1 is a small molecule activator of telomerase reverse transcriptase (TERT). TERT activator-1 promotes TERT transcription through the MEK/ERK/AP-1 signaling cascade. TERT activator-1 promotes adult neurogenesis and enhances neuromuscular function. TERT activator-1 reduces cellular senescence and systemic inflammation in aged mice, and can be used in the study of aging.

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TERT activator-1 Chemical Structure

TERT activator-1 Chemical Structure

CAS No. : 666699-46-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
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10 mg In-stock
25 mg In-stock
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Based on 1 publication(s) in Google Scholar

TERT activator-1 Related Antibodies

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c-Fos c-Jun

  • Biological Activity

  • Purity & Documentation

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Description

TERT activator-1 is a small molecule activator of telomerase reverse transcriptase (TERT). TERT activator-1 promotes TERT transcription through the MEK/ERK/AP-1 signaling cascade. TERT activator-1 promotes adult neurogenesis and enhances neuromuscular function. TERT activator-1 reduces cellular senescence and systemic inflammation in aged mice, and can be used in the study of aging[1].

IC50 & Target[1]

MEK1

 

MEK2

 

ERK1

 

ERK2

 

In Vitro

TERT activator-1 (0-10 μM; 0-4 h) specifically activates TERT transcription in MRC-5 cells via the MEK/ERK/AP-1 pathway and can alter chromatin status in the TERT promoter region[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TERT activator-1 Related Antibodies

Real Time qPCR[1]

Cell Line: MRC-5 human fibroblasts cells
Concentration: 0, 0.05, 0.1, 0.5, 1, 5 and 10 μM
Incubation Time: 4 h
Result: Reduced the mRNA levels of TERT.
In Vivo

TERT activator-1 (6 mg/kg; intraperitoneal injection; 1-6 weeks) alleviates aging and promotes adult neurogenesis in naturally aging mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice aged 10-12 weeks old[1]
Dosage: 6 mg/kg
Administration: Intraperitoneal injection (i.p.); 1 week
Result: Significantly increased the level of TERT protein.
Reduced aging genetic traits including cell-cycle arrest and PML body.
Suppressed p16Ink4a and other senescence-associated secretory phenotype (SASP) components.
Induced signatures of organism growth and natural killer cell activation.
Increased in the levels of mBDNF in adult hippocampus.
Animal Model: Naturally aged C57BL/6 mice aged 20-21 months old[1]
Dosage: 6 mg/kg
Administration: Intraperitoneal injection (i.p.); 3 times a week; 6 months
Result: Improves hippocampal-dependent cognitive function.
Enhances neuromuscular function.
Did not result in any overt adverse consequences.
Molecular Weight

331.77

Formula

C14H12ClF2NO2S
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=S(NC1=CC=C(C(Cl)=C1)F)(C2=CC(C)=C(F)C(C)=C2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (301.41 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0141 mL 15.0707 mL 30.1414 mL
5 mM 0.6028 mL 3.0141 mL 6.0283 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (7.54 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.50%

COA (249 KB) HNMR (219 KB) RP-HPLC (251 KB) LCMS (143 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0141 mL 15.0707 mL 30.1414 mL 75.3534 mL
5 mM 0.6028 mL 3.0141 mL 6.0283 mL 15.0707 mL
10 mM 0.3014 mL 1.5071 mL 3.0141 mL 7.5353 mL
15 mM 0.2009 mL 1.0047 mL 2.0094 mL 5.0236 mL
20 mM 0.1507 mL 0.7535 mL 1.5071 mL 3.7677 mL
25 mM 0.1206 mL 0.6028 mL 1.2057 mL 3.0141 mL
30 mM 0.1005 mL 0.5024 mL 1.0047 mL 2.5118 mL
40 mM 0.0754 mL 0.3768 mL 0.7535 mL 1.8838 mL
50 mM 0.0603 mL 0.3014 mL 0.6028 mL 1.5071 mL
60 mM 0.0502 mL 0.2512 mL 0.5024 mL 1.2559 mL
80 mM 0.0377 mL 0.1884 mL 0.3768 mL 0.9419 mL
100 mM 0.0301 mL 0.1507 mL 0.3014 mL 0.7535 mL
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TERT activator-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TERT activator-1666699-46-3TERT activator1TERT activator 1MEKERKAP-1Mitogen-activated protein kinase kinaseMAPKKMAP2KExtracellular signal regulated kinasesActivator Protein 1TERT activatorAgingAdult neurogenesisInhibitorinhibitorinhibit

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