Tetramethylthiuram monosulfide (Synonyms: TMTM)

Cat. No.: HY-W020246 Purity: 99.28%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetramethylthiuram monosulfide (TMTM) is an active compound. Tetramethylthiuram monosulfide (TMTM) can be used for the research of rubber and various biochemical studies.

For research use only. We do not sell to patients.

Tetramethylthiuram monosulfide Chemical Structure

CAS No. : 97-74-5

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25 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
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Purity & Documentation
References
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Description

Tetramethylthiuram monosulfide (TMTM) is an active compound. Tetramethylthiuram monosulfide (TMTM) can be used for the research of rubber and various biochemical studies^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	> 5000 μM Compound: 9	Cytotoxicity against human HeLa cells after 24 hrs by MTT assay Cytotoxicity against human HeLa cells after 24 hrs by MTT assay	[PMID: 29216562]

Molecular Weight

208.37

Formula

C₆H₁₂N₂S₃

CAS No.

97-74-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

S=C(SC(N(C)C)=S)N(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (1199.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.7992 mL	23.9958 mL	47.9916 mL
5 mM	0.9598 mL	4.7992 mL	9.5983 mL
10 mM	0.4799 mL	2.3996 mL	4.7992 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (9.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (9.98 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.28%

Select Batch:

Data Sheet (269 KB) SDS (537 KB)

COA (245 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Coleman, et al. Vibrational spectra of thiuram sulfides. Normal coordinate analysis of tetramethylthiuram mono-, di-, and tetrasulfides. Journal of Polymer Science, Polymer Physics Edition.Volume: 12.Issue: 5.Pages: 1001-13.Journal.1974.

[2]. Yingtao Sun, et al. Synergistic Effect of Copper Sulfate and Tetramethylthiuram Monosulfide Induced Metal-Coordination Cross-Linking in Nitrile–Butadiene Rubber. 25 April 2021.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.7992 mL	23.9958 mL	47.9916 mL	119.9789 mL
	5 mM	0.9598 mL	4.7992 mL	9.5983 mL	23.9958 mL
	10 mM	0.4799 mL	2.3996 mL	4.7992 mL	11.9979 mL
	15 mM	0.3199 mL	1.5997 mL	3.1994 mL	7.9986 mL
	20 mM	0.2400 mL	1.1998 mL	2.3996 mL	5.9989 mL
	25 mM	0.1920 mL	0.9598 mL	1.9197 mL	4.7992 mL
	30 mM	0.1600 mL	0.7999 mL	1.5997 mL	3.9993 mL
	40 mM	0.1200 mL	0.5999 mL	1.1998 mL	2.9995 mL
	50 mM	0.0960 mL	0.4799 mL	0.9598 mL	2.3996 mL
	60 mM	0.0800 mL	0.3999 mL	0.7999 mL	1.9996 mL
	80 mM	0.0600 mL	0.2999 mL	0.5999 mL	1.4997 mL
	100 mM	0.0480 mL	0.2400 mL	0.4799 mL	1.1998 mL

Tetramethylthiuram monosulfide Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tetramethylthiuram monosulfide97-74-5TMTMOthersactive compoundbiochemical studiesInhibitorinhibitorinhibit

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