Tetrazine-Ph-acid

Name: Tetrazine-Ph-acid
Brand: MedChemExpress
SKU: HY-124480
Rating: 4.5 (1 reviews)

Cat. No.: HY-124480 Purity: 98.79%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetrazine-Ph-acid is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs. Tetrazine-Ph-acid is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

For research use only. We do not sell to patients.

Tetrazine-Ph-acid Chemical Structure

CAS No. : 1380500-92-4

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
25 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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PEG Linkers Alkyl-Chain Linkers Alkyl/ether Linkers

Biological Activity
Purity & Documentation
References
Customer Review

Description

Tetrazine-Ph-acid is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs^[1]. Tetrazine-Ph-acid is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups.

IC₅₀ & Target

Alkyl-Chain

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

216.20

Formula

C₁₀H₈N₄O₂

CAS No.

1380500-92-4

Appearance

Solid

Color

Pink to red

SMILES

OC(CC1=CC=C(C2=NN=CN=N2)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (231.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.6253 mL	23.1267 mL	46.2535 mL
5 mM	0.9251 mL	4.6253 mL	9.2507 mL
10 mM	0.4625 mL	2.3127 mL	4.6253 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.79%

Select Batch:

Data Sheet (271 KB) SDS (466 KB)

COA (246 KB) RP-HPLC (214 KB) LCMS (270 KB)

Handling Instructions (2659 KB)

References

[1]. An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.6253 mL	23.1267 mL	46.2535 mL	115.6337 mL
	5 mM	0.9251 mL	4.6253 mL	9.2507 mL	23.1267 mL
	10 mM	0.4625 mL	2.3127 mL	4.6253 mL	11.5634 mL
	15 mM	0.3084 mL	1.5418 mL	3.0836 mL	7.7089 mL
	20 mM	0.2313 mL	1.1563 mL	2.3127 mL	5.7817 mL
	25 mM	0.1850 mL	0.9251 mL	1.8501 mL	4.6253 mL
	30 mM	0.1542 mL	0.7709 mL	1.5418 mL	3.8545 mL
	40 mM	0.1156 mL	0.5782 mL	1.1563 mL	2.8908 mL
	50 mM	0.0925 mL	0.4625 mL	0.9251 mL	2.3127 mL
	60 mM	0.0771 mL	0.3854 mL	0.7709 mL	1.9272 mL
	80 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4454 mL
	100 mM	0.0463 mL	0.2313 mL	0.4625 mL	1.1563 mL