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  4. THAM hydrochloride (≥99%, reagent grade)

THAM hydrochloride (≥99%, reagent grade)  (Synonyms: Tris HCl (≥99%, reagent grade); Tris hydrochloride (≥99%, reagent grade))

Cat. No.: HY-D0227F Purity: ≥99.0%
COA Handling Instructions

Molarity Calculator

THAM hydrochloride (≥99%, reagent grade), also known as Tris-HCl, is a buffer commonly used in various biochemical and molecular biology applications to maintain a stable pH environment. Tris-HCl has unique chemical properties that allow it to resist changes in pH when acidic or basic substances are added, which makes it useful for stabilizing biological samples or reagents. It is commonly used in electrophoresis and protein purification procedures.

For research use only. We do not sell to patients.

THAM hydrochloride (≥99%, reagent grade) Chemical Structure

THAM hydrochloride (≥99%, reagent grade) Chemical Structure

CAS No. : 1185-53-1

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Based on 1 publication(s) in Google Scholar

THAM hydrochloride (≥99%, reagent grade) Related Antibodies

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  • Purity & Documentation

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Description

THAM hydrochloride (≥99%, reagent grade), also known as Tris-HCl, is a buffer commonly used in various biochemical and molecular biology applications to maintain a stable pH environment. Tris-HCl has unique chemical properties that allow it to resist changes in pH when acidic or basic substances are added, which makes it useful for stabilizing biological samples or reagents. It is commonly used in electrophoresis and protein purification procedures.

In Vitro

THAM hydrochloride (≥99%, reagent grade) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

THAM hydrochloride (≥99%, reagent grade) Related Antibodies
Molecular Weight

157.60

Formula

C4H12ClNO3
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

OCC(CO)(N)CO.[H]Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 233.33 mg/mL (1480.52 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (317.26 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.3452 mL 31.7259 mL 63.4518 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (13.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (13.20 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥99.0%

COA (249 KB) HNMR (239 KB) MS (81 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 6.3452 mL 31.7259 mL 63.4518 mL 158.6294 mL
5 mM 1.2690 mL 6.3452 mL 12.6904 mL 31.7259 mL
10 mM 0.6345 mL 3.1726 mL 6.3452 mL 15.8629 mL
15 mM 0.4230 mL 2.1151 mL 4.2301 mL 10.5753 mL
20 mM 0.3173 mL 1.5863 mL 3.1726 mL 7.9315 mL
25 mM 0.2538 mL 1.2690 mL 2.5381 mL 6.3452 mL
30 mM 0.2115 mL 1.0575 mL 2.1151 mL 5.2876 mL
40 mM 0.1586 mL 0.7931 mL 1.5863 mL 3.9657 mL
50 mM 0.1269 mL 0.6345 mL 1.2690 mL 3.1726 mL
60 mM 0.1058 mL 0.5288 mL 1.0575 mL 2.6438 mL
80 mM 0.0793 mL 0.3966 mL 0.7931 mL 1.9829 mL
100 mM 0.0635 mL 0.3173 mL 0.6345 mL 1.5863 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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THAM hydrochloride (≥99%, reagent grade) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

THAM hydrochloride (≥99%, reagent grade)1185-53-1Tris HCl (≥99%, reagent grade) Tris hydrochloride (≥99%, reagent grade)Biochemical Assay Reagentsbiochemical assayreagentInhibitorinhibitorinhibit

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