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Tiron is a non-toxic chelator of a variety of metals. Tiron is cell permeable analog of vitamin E and function as hydroxyl radical and superoxide scavenger. Tiron is an orally active antioxidant. Tiron can be used to alleviate acute metal overload in animals.

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Tiron Chemical Structure

Tiron Chemical Structure

CAS No. : 149-45-1

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Tiron Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tiron is a non-toxic chelator of a variety of metals. Tiron is cell permeable analog of vitamin E and function as hydroxyl radical and superoxide scavenger. Tiron is an orally active antioxidant. Tiron can be used to alleviate acute metal overload in animals[1][2][3].

In Vitro

Tiron (10 mM) protects Chinese hamster V79 cells against H2O2-induced cytotoxicity[1].
Tiron (0-20 mM) protects supercoiled DNA from metal-mediated superoxide-dependent strand breaks[1].
Tiron (50 nM-200 nM, 48 h) inhibits HG-induced neonatal rat cardiomyocytes apoptosis[3].
Tiron (50 nM-200 nM, 48 h) reduces intracellular osteopontin in neonatal rat cardiomyocytes[3].
Tiron (0.2 mM, 2 h) inhibits UVB-induced up-regulation of MMP-1 and MMP-3 in HDFs[4].
Tiron (0.7 mM, 48 h) increases the percentage of PT4 cells in both the S and G2/M phases[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tiron Related Antibodies

Cell Cycle Analysis[5]

Cell Line: PT4 cells
Concentration: 0.7 mM
Incubation Time: 48 h
Result: Increased the percentage of PT4 cells in S and G2/M phases, along with a reduction of cells in the G0/G1 phase.
In Vivo

Tiron (200 mg/kg, oral gavage) ameliorates oxidative stress and inflammation in a murine model of airway remodeling[2].
Tiron (300 mg/kg, i.p., daily for two weeks) alleviated apoptosis of the left ventricular cardiomyocytes in STZ-induced diabetic mice[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice challenged with Ovalbumin (OVA) aerosol for 8 weeks[2]
Dosage: 200 mg/kg
Administration: Oral gavage.
Result: Inhibited levels of NOx, IL-13 and TGF-β1, and immunoreactivity of NF-κB.
Animal Model: STZ-induced diabetic mice[3]
Dosage: 300 mg/kg
Administration: i.p., daily for two weeks.
Result: Reduced the levels of total oxidized proteins and advanced glycation end products (AGEs)-related proteins.
Inhibited cardiac myocyte apoptosis.
Decreased PKCδ localization on plasma membrane.
Molecular Weight

314.20

Formula

C6H4Na2O8S2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=S(C1=C(O)C(O)=CC(S(=O)([O-])=O)=C1)([O-])=O.[Na+].[Na+]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (265.21 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1827 mL 15.9134 mL 31.8269 mL
5 mM 0.6365 mL 3.1827 mL 6.3654 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (6.62 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.94%

SDS (393 KB)

COA (252 KB) HNMR (248 KB) LCMS (86 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.1827 mL 15.9134 mL 31.8269 mL 79.5672 mL
5 mM 0.6365 mL 3.1827 mL 6.3654 mL 15.9134 mL
10 mM 0.3183 mL 1.5913 mL 3.1827 mL 7.9567 mL
15 mM 0.2122 mL 1.0609 mL 2.1218 mL 5.3045 mL
20 mM 0.1591 mL 0.7957 mL 1.5913 mL 3.9784 mL
25 mM 0.1273 mL 0.6365 mL 1.2731 mL 3.1827 mL
30 mM 0.1061 mL 0.5304 mL 1.0609 mL 2.6522 mL
40 mM 0.0796 mL 0.3978 mL 0.7957 mL 1.9892 mL
50 mM 0.0637 mL 0.3183 mL 0.6365 mL 1.5913 mL
60 mM 0.0530 mL 0.2652 mL 0.5304 mL 1.3261 mL
80 mM 0.0398 mL 0.1989 mL 0.3978 mL 0.9946 mL
100 mM 0.0318 mL 0.1591 mL 0.3183 mL 0.7957 mL
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Tiron Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tiron149-45-1Biochemical Assay ReagentsApoptosisMetaloxidativeV79DNA breakmetal chelatorairway remodelingasthmacardiomyocytesantioxidantInhibitorinhibitorinhibit

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