2. Apoptosis Immunology/Inflammation
  3. Apoptosis TNF Receptor Interleukin Related
  4. Tributyrin

Tributyrin  (Synonyms: Glyceryl tributyrate)

Cat. No.: HY-W011404 Purity: 98.91%
SDS
COA
Handling Instructions Technical Support

Tributyrin (Glyceryl tributyrate), a neutral short-chain fatty acid triglyceride, is a stable and rapidly absorbed proagent of Butyric Acid. Tributyrin diffuses through biological membranes and is metabolized by intracellular lipases, releasing effective butyrate directly into the cell in vivo. Tributyrin has potent antiproliferative, proapoptotic and differentiation-inducing effects.

For research use only. We do not sell to patients.

Tributyrin Chemical Structure

Tributyrin Chemical Structure

CAS No. : 60-01-5

Size Price Stock Quantity
Solvent
10 mM * 1 mL in DMSO In-stock
Solvent
5 mL In-stock
10 mL   Get quote  
50 mL   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Tributyrin:

Tributyrin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Tributyrin

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Tributyrin (Glyceryl tributyrate), a neutral short-chain fatty acid triglyceride, is a stable and rapidly absorbed proagent of Butyric Acid. Tributyrin diffuses through biological membranes and is metabolized by intracellular lipases, releasing effective butyrate directly into the cell in vivo. Tributyrin has potent antiproliferative, proapoptotic and differentiation-inducing effects[1].

IC50 & Target

IL-1β

 

In Vitro

Tributyrin (0.8 mM, 48 hours) induces significant growth inhibition in PC-3 cells[3].
Tributyrin (0.5-5 mM, 48 hours) causes G1 cell cycle arrest in LNCaP cells[3].
Tributyrin (0.1-5 mM, 48 hours) induces higher apoptosis rates in PC-3 and TSU-PR1 cells[3].
Tributyrin (1-5 mM, 48 hours) induces prostate-specific antigen expression in LNCaP cells, suggesting its differentiation effect[3].
Tributyrin (1 mM, 24 hours) directly inhibits the production of TNF-α and IL-1β in RAW 264.7 cells under LPS stimulation[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tributyrin Related Antibodies

Cell Proliferation Assay[3]

Cell Line: LNCaP, PC-3, TSU-PR1 cells (human prostate cancer)
Concentration: 0.1-5 mM
Incubation Time: 48 hours
Result: Exhibited significant growth inhibition in all three cell lines, with IC50 of 0.8 mM in PC-3 cells, 1.2 mM in TSU-PR1 cells, and 3.1 mM in LNCaP cells.

Cell Cycle Analysis[3]

Cell Line: LNCaP cells (human prostate cancer)
Concentration: 0.5, 2, 5 mM
Incubation Time: 48 hours
Result: Significantly reduced the fraction of LNCaP cells in the S-phase and caused strong accumulation in the G1 phase.
In Vivo

Tributyrin (2.0 g/kg, p.o., three times per week for 6 weeks) improves the metabolic and inflammatory states in high-fat diet-induced obese mice[4].
Tributyrin (0.3 g/kg, p.o., once daily for 11 days) significantly improves gut microbiota dysbiosis and intestinal injury in a Ceftriaxone (HY-B0712)-induced gut dysfunction mouse model[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: High-fat diet (HFD)-induced obese mice (C57BL/6)[4]
Dosage: 2.0 g/kg
Administration: Oral gavage (p.o.), 3 times per week, with 48-hour intervals, for 6 weeks
Result: Led to lower body weight gain compared to control, improved glucose tolerance, increased insulin sensitivity, and reduced liver triglyceride content.
Animal Model: Ceftriaxone (HY-B0712)-induced gut microbiota dysbiosis in C57BL/6 mice
Dosage: 0.3 g/kg, 3 g/kg
Administration: Oral gavage (p.o.), once daily, for 11 days
Result: Improved body weight, reduced diarrhea, and less intestinal tissue damage (0.3 g/kg).
Clinical Trial
Molecular Weight

302.36

Formula

C15H26O6
CAS No.
Appearance

Liquid (Density: 1 g/ml)

Color

Colorless to light yellow

SMILES

CCCC(OCC(OC(CCC)=O)COC(CCC)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (330.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3073 mL 16.5366 mL 33.0732 mL
5 mM 0.6615 mL 3.3073 mL 6.6146 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.91%

SDS (393 KB)

COA (244 KB) HNMR (247 KB) RP-HPLC (212 KB) MS (68 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3073 mL 16.5366 mL 33.0732 mL 82.6829 mL
5 mM 0.6615 mL 3.3073 mL 6.6146 mL 16.5366 mL
10 mM 0.3307 mL 1.6537 mL 3.3073 mL 8.2683 mL
15 mM 0.2205 mL 1.1024 mL 2.2049 mL 5.5122 mL
20 mM 0.1654 mL 0.8268 mL 1.6537 mL 4.1341 mL
25 mM 0.1323 mL 0.6615 mL 1.3229 mL 3.3073 mL
30 mM 0.1102 mL 0.5512 mL 1.1024 mL 2.7561 mL
40 mM 0.0827 mL 0.4134 mL 0.8268 mL 2.0671 mL
50 mM 0.0661 mL 0.3307 mL 0.6615 mL 1.6537 mL
60 mM 0.0551 mL 0.2756 mL 0.5512 mL 1.3780 mL
80 mM 0.0413 mL 0.2067 mL 0.4134 mL 1.0335 mL
100 mM 0.0331 mL 0.1654 mL 0.3307 mL 0.8268 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Tributyrin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tributyrin60-01-5Glyceryl tributyrateApoptosisTNF ReceptorInterleukin RelatedTumor Necrosis Factor ReceptorTNFRILVitamin DCaco-2 cellprodrugantiproliferativeproapoptoticcell differentiationfatty acid triglyceride ApoptosisMiaPaca-2Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Tributyrin
Cat. No.:
HY-W011404
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.