Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride

Name: Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride
Brand: MedChemExpress
SKU: HY-W074143
Rating: 4.5 (1 reviews)

Cat. No.: HY-W074143 Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride is an oxygen-sensitive fluorescent indicator widely used as a probe for luminescence detection and quantitative oxygen determination.

For research use only. We do not sell to patients.

Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride Chemical Structure

CAS No. : 36309-88-3

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Free Sample (0.1 - 0.5 mg)		Apply Now
250 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
1 g	USD 118	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride

•ACS Appl Nano Mater. 2024 Jun 26.

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Biological Activity
Purity & Documentation
References
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Description

Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride is an oxygen-sensitive fluorescent indicator widely used as a probe for luminescence detection and quantitative oxygen determination^[1].

Molecular Weight

1169.17

Formula

C₇₂H₄₈Cl₂N₆Ru

CAS No.

36309-88-3

Appearance

Solid

Color

Orange to red

Emission (Em)

613

Excitation (Ex)

455

SMILES

C1(C=C2)=C(C3=C2C(C4=CC=CC=C4)=CC=[N]3[Ru+2]567([N]8=CC=C(C9=CC=CC=C9)C%10=C8C%11=[N]7C=CC(C%12=CC=CC=C%12)=C%11C=C%10)[N]%13=CC=C(C%14=CC=CC=C%14)C%15=C%13C%16=[N]6C=CC(C%17=CC=CC=C%17)=C%16C=C%15)[N]5=CC=C1C%18=CC=CC=C%18.[2Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (21.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 8.33 mg/mL (7.12 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.8553 mL	4.2765 mL	8.5531 mL
5 mM	0.1711 mL	0.8553 mL	1.7106 mL
10 mM	0.0855 mL	0.4277 mL	0.8553 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (1.07 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (0.71 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.83 mg/mL (0.71 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (258 KB) HNMR (294 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Hao Xu, et al. A real-time ratiometric method for the determination of molecular oxygen inside living cells using sol-gel-based spherical optical nanosensors with applications to rat C6 glioma. Anal Chem. 2001 Sep 1;73(17):4124-33. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	0.8553 mL	4.2765 mL	8.5531 mL	21.3827 mL
Ethanol / DMSO	5 mM	0.1711 mL	0.8553 mL	1.7106 mL	4.2765 mL
DMSO	10 mM	0.0855 mL	0.4277 mL	0.8553 mL	2.1383 mL
	15 mM	0.0570 mL	0.2851 mL	0.5702 mL	1.4255 mL
	20 mM	0.0428 mL	0.2138 mL	0.4277 mL	1.0691 mL