Tubulin inhibitor 30

Name: Tubulin inhibitor 30
Brand: MedChemExpress
SKU: HY-151879
Rating: 4.5 (1 reviews)

Cat. No.: HY-151879 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tubulin inhibitor 30 (Compound 15) is a tubulin assembly inhibitor with an IC₅₀ of 0.52 μM. Tubulin inhibitor 30 can induce ferroptosis.

For research use only. We do not sell to patients.

Tubulin inhibitor 30 Chemical Structure

CAS No. : 2873383-67-4

Size	Price	Stock	Quantity
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 320	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1050	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1680	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Tubulin inhibitor 30 (Compound 15) is a tubulin assembly inhibitor with an IC₅₀ of 0.52 μM. Tubulin inhibitor 30 can induce ferroptosis^[1].

IC₅₀ & Target

IC50: 0.52 μM (Tubulin)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
MCF7	IC₅₀	4 nM Compound: 15	Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by trypan blue exclusion assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by trypan blue exclusion assay	[PMID: 36395526]
OVCAR-3	IC₅₀	2.85 nM Compound: 15	Cytotoxicity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay Cytotoxicity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay	[PMID: 36395526]
U-87MG ATCC	IC₅₀	10.06 nM Compound: 15	Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay	[PMID: 36395526]

In Vitro

Tubulin inhibitor 30 (Compound 15) (0-100 nM; 48 h) inhibits the viability of MCF-7, U-87 MG and OVCAR-3 cells^[1].
Tubulin inhibitor 30 is metabolically stable when incubated with human liver microsomes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	MCF-7, U-87 MG and OVCAR-3 cells
Concentration:	0-100 nM
Incubation Time:	48 h
Result:	Inhibited the cell viability with IC₅₀s of 4.0, 10.06 and 2.852 nM against MCF-7, U-87 MG and OVCAR-3 cells, respectively.

Western Blot Analysis^[1]

Cell Line:	U-87 MG and OVCAR-3 cells
Concentration:	0-100 nM
Incubation Time:	48 h
Result:	Induced a significant increase of cleaved PARP.

In Vivo

Tubulin inhibitor 30 (Compound 15) (25mg/kg; i.p.; every 2 days) inhibits the in vivo tumorigenicity of the human GBM cell line U-87 MG and the human OC cell line SKOV-3, and causes oxidative stress injury and Fe²⁺ accumulation in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/C^nu/nu mice, U-87 MG or SKOV-3 model^[1]
Dosage:	25 mg/kg
Administration:	Intraperitoneal injection, every 2 days
Result:	Inhibited tumorigenicity and tumor volume. Caused oxidative stress injury and Fe²⁺ accumulation.

Molecular Weight

405.40

Formula

C₂₂H₁₉N₃O₅

CAS No.

2873383-67-4

Appearance

Solid

Color

White to off-white

SMILES

COC1=CC(C(C2=CN(C=C2C3=CC=CO3)C4=NC=CC=N4)=O)=CC(OC)=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (12.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4667 mL	12.3335 mL	24.6670 mL
5 mM	0.4933 mL	2.4667 mL	4.9334 mL
10 mM	0.2467 mL	1.2333 mL	2.4667 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.23 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (280 KB) SDS (251 KB)

COA (259 KB) HNMR (254 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Puxeddu M, et al. Induction of Ferroptosis in Glioblastoma and Ovarian Cancers by a New Pyrrole Tubulin Assembly Inhibitor. J Med Chem. 2022 Nov 17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4667 mL	12.3335 mL	24.6670 mL	61.6675 mL
	5 mM	0.4933 mL	2.4667 mL	4.9334 mL	12.3335 mL
	10 mM	0.2467 mL	1.2333 mL	2.4667 mL	6.1667 mL