VH285-PEG4-C4-Cl (Synonyms: HaloPROTAC 3)

Name: VH285-PEG4-C4-Cl
Brand: MedChemExpress
SKU: HY-111997
Rating: 4.5 (1 reviews)

Cat. No.: HY-111997 Purity: 96.49%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

VH285-PEG4-C4-Cl (HaloPROTAC 3) is a conjugate of ligands for E3 and 16-atom-length linker. The connector of linker is Halogen group. VH285-PEG4-C4-Cl incorporates the VH285 based VHL ligand and an alkyl/ether-based linker. VH285-PEG4-C4-Cl is a highly potent and efficacious degrader of GFP-HaloTag7 with a DC₅₀ of 19 nM. VH285-PEG4-C4-Cl is able to induce 90 % degradation of GFP-Halotag at 625 nM. VH285-PEG4-C4-Cl binds to VHL with an IC₅₀ of 0.54 μM.

For research use only. We do not sell to patients.

VH285-PEG4-C4-Cl Chemical Structure

CAS No. : 1799506-07-2

Size	Price	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 484	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 484	In-stock	Estimated Time of Arrival: December 31
Liquid
5 mg	USD 340	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1950	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3120	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Purity & Documentation
References
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Description

IC₅₀ & Target

VHL

In Vitro

Treatment with 500 nM VH285-PEG4-C4-Cl (HaloPROTAC 3) is able to induce nearly complete knockdown of both HaloTag7-ERK1 and HaloTag7-MEK1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

799.42

Formula

C₄₁H₅₅ClN₄O₈S

CAS No.

1799506-07-2

Appearance

Liquid (Density: 1.250±0.06 g/cm³)

Color

Colorless to off-white

SMILES

CC(N=CS1)=C1C2=CC(OCCOCCOCCOCCCCCCCl)=C(CNC([C@@H]3C[C@@H](O)CN3C([C@@H](N4CC(C=CC=C5)=C5C4=O)C(C)C)=O)=O)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (125.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2509 mL	6.2545 mL	12.5091 mL
5 mM	0.2502 mL	1.2509 mL	2.5018 mL
10 mM	0.1251 mL	0.6255 mL	1.2509 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.13 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (3.13 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (246 KB)

Handling Instructions (2659 KB)

References

[1]. Buckley DL, et al. HaloPROTACS: Use of Small Molecule PROTACs to Induce Degradation of HaloTag Fusion Proteins. ACS Chem Biol. 2015 Aug 21;10(8):1831-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2509 mL	6.2545 mL	12.5091 mL	31.2727 mL
	5 mM	0.2502 mL	1.2509 mL	2.5018 mL	6.2545 mL
	10 mM	0.1251 mL	0.6255 mL	1.2509 mL	3.1273 mL
	15 mM	0.0834 mL	0.4170 mL	0.8339 mL	2.0848 mL
	20 mM	0.0625 mL	0.3127 mL	0.6255 mL	1.5636 mL
	25 mM	0.0500 mL	0.2502 mL	0.5004 mL	1.2509 mL
	30 mM	0.0417 mL	0.2085 mL	0.4170 mL	1.0424 mL
	40 mM	0.0313 mL	0.1564 mL	0.3127 mL	0.7818 mL
	50 mM	0.0250 mL	0.1251 mL	0.2502 mL	0.6255 mL
	60 mM	0.0208 mL	0.1042 mL	0.2085 mL	0.5212 mL
	80 mM	0.0156 mL	0.0782 mL	0.1564 mL	0.3909 mL
	100 mM	0.0125 mL	0.0625 mL	0.1251 mL	0.3127 mL

VH285-PEG4-C4-Cl Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VH285-PEG4-C4-Cl1799506-07-2HaloPROTAC 3HaloPROTAC3HaloPROTAC-3E3 Ligase Ligand-Linker ConjugatesInhibitorinhibitorinhibit

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VH285-PEG4-C4-Cl (Synonyms: HaloPROTAC 3)

Customer Review

VH285-PEG4-C4-Cl Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All E3 Ligase Ligand-Linker Conjugates Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

VH285-PEG4-C4-Cl Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

VH285-PEG4-C4-Cl Related Classifications

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