2. Apoptosis Metabolic Enzyme/Protease
  3. Apoptosis Endogenous Metabolite
  4. Violacein

Violacein, a secondary metabolite produced by several microorganisms, possesses potent anticancer and low side effects. Violacein possesses antioxidant properties. Apoptosis inducer.

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Violacein Chemical Structure

Violacein Chemical Structure

CAS No. : 548-54-9

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Based on 1 publication(s) in Google Scholar

Violacein Related Antibodies

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  • Purity & Documentation

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Description

Violacein, a secondary metabolite produced by several microorganisms, possesses potent anticancer and low side effects. Violacein possesses antioxidant properties. Apoptosis inducer[1][2].

IC50 & Target

Microbial Metabolite

 

Cellular Effect
Cell Line Type Value Description References
Caco-2 IC50
> 1.5 μM
Compound: violacein
Cytotoxicity against Homo sapiens (human) Caco2 cells after 24 to 48 hr by MTT assay
Cytotoxicity against Homo sapiens (human) Caco2 cells after 24 to 48 hr by MTT assay
10.1007/s00044-011-9654-9
DLD-1 IC50
> 1.5 μM
Compound: violacein
Cytotoxicity against Homo sapiens (human) DLD1 cells after 24 to 48 hr by MTT assay
Cytotoxicity against Homo sapiens (human) DLD1 cells after 24 to 48 hr by MTT assay
10.1007/s00044-011-9654-9
HCT-116 IC50
> 1.5 μM
Compound: violacein
Cytotoxicity against Homo sapiens (human) HCT116 cells after 24 to 48 hr by MTT assay
Cytotoxicity against Homo sapiens (human) HCT116 cells after 24 to 48 hr by MTT assay
10.1007/s00044-011-9654-9
HT-29 IC50
> 1.5 μM
Compound: violacein
Cytotoxicity against Homo sapiens (human) HT-29 cells after 24 to 48 hr by MTT assay
Cytotoxicity against Homo sapiens (human) HT-29 cells after 24 to 48 hr by MTT assay
10.1007/s00044-011-9654-9
In Vitro

Violacein (0.25-3 µM; 24h; HCT116 and HT29 cells) possesses anticancer activity in both 2D and 3D cell models[1].
Violacein decreases RTKs expression and disturbs signaling pathways in CRC cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Violacein Related Antibodies

Cell Viability Assay[1].

Cell Line: HCT116 and HT29 (1.8 × 104 cells/well-100 µL) cells.
Concentration: 0.25, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 µM.
Incubation Time: 24h.
Result: In the 2D culture model, the violacein treatment reduced the cell viability, since there was a decrease in formazan production in the HT29 and HCT116 cell lines. Moreover, HT29 was more sensitive to violacein, as evidenced by the IC50 value (0.6 μM) compared to HCT116 (1.2 μM).
Molecular Weight

343.34

Formula

C20H13N3O3
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

O=C1/C(C2=CC=CC=C2N1)=C3C=C(C(C4=C5)=CNC4=CC=C5O)NC\3=O
Structure Classification
Initial Source

Chromobacterium violaceum
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (29.13 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9126 mL 14.5628 mL 29.1256 mL
5 mM 0.5825 mL 2.9126 mL 5.8251 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9126 mL 14.5628 mL 29.1256 mL 72.8141 mL
5 mM 0.5825 mL 2.9126 mL 5.8251 mL 14.5628 mL
10 mM 0.2913 mL 1.4563 mL 2.9126 mL 7.2814 mL
15 mM 0.1942 mL 0.9709 mL 1.9417 mL 4.8543 mL
20 mM 0.1456 mL 0.7281 mL 1.4563 mL 3.6407 mL
25 mM 0.1165 mL 0.5825 mL 1.1650 mL 2.9126 mL
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Violacein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Violacein548-54-9ApoptosisEndogenous MetaboliteAnticancerMicroorganisms Secondary Metaboliteantioxidantapoptosiscolon cancer,Inhibitorinhibitorinhibit

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