VU591

Name: VU591
Brand: MedChemExpress
SKU: HY-108585A
Rating: 4.5 (1 reviews)

Cat. No.: HY-108585A Purity: 98.39%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

VU591 is a potent, selective renal outer medullary potassium channel (ROMK or Kir1.1) inhibitor, with an IC₅₀ of 0.24 μM. VU591 can be used for neurological research with HY-108585 (the equivalent of VU591 hydrochloride).

For research use only. We do not sell to patients.

VU591 Chemical Structure

CAS No. : 1222810-74-3

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 99	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 99	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 830	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of VU591:

VU591 hydrochloride Get quote

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References
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Description

IC₅₀ & Target

IC50: 0.24 μM (ROMK)^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	0.24 μM Compound: 2	Inhibition of ROMK S44D mutant expressed in HEK293 cells assessed as inhibition of thallium efflux incubated for 20 mins prior to thallium addition measured after 7 to 12 secs by fluorescence assay Inhibition of ROMK S44D mutant expressed in HEK293 cells assessed as inhibition of thallium efflux incubated for 20 mins prior to thallium addition measured after 7 to 12 secs by fluorescence assay	[PMID: 24900480]
HEK293	IC₅₀	240 nM Compound: 3; VU591	Inhibition of rat ROMK1 expressed in HEK293 cells by Tl+ flux assay Inhibition of rat ROMK1 expressed in HEK293 cells by Tl+ flux assay	[PMID: 31034224]

In Vitro

VU591 is a selective ROMK inhibitor and a ROMK channel pore blocker. VU591 can bind serum protein and has high metabolic stability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

VU591 (i.c.v.; 1.842 μg) significantly decreases the immobile time in TST^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ICR mice^[2]
Dosage:	1.842 μg
Administration:	i.c.v.; 1.842 μg;
Result:	Showed antidepressive effect in the tail suspension test (TST).

Molecular Weight

368.30

Formula

C₁₆H₁₂N₆O₅

CAS No.

1222810-74-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+]([O-])C1=CC(NC(COCC2=NC3=C(N2)C=C([N+]([O-])=O)C=C3)=N4)=C4C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (169.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7152 mL	13.5759 mL	27.1518 mL
5 mM	0.5430 mL	2.7152 mL	5.4304 mL
10 mM	0.2715 mL	1.3576 mL	2.7152 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.65 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.39%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB) HNMR (209 KB) LCMS (120 KB)

Handling Instructions (2659 KB)

References

[1]. Bhave G, et al. Development of a selective small-molecule inhibitor of Kir1.1, the renal outer medullary potassium channel. Mol Pharmacol. 2011 Jan;79(1):42-50. [Content Brief]

[2]. Masayoshi Okada, et al. Antidepressive effect of an inward rectifier K+ channel blocker peptide, tertiapin-RQ. PLoS One. 2020 Nov 13;15(11):e0233815. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7152 mL	13.5759 mL	27.1518 mL	67.8794 mL
	5 mM	0.5430 mL	2.7152 mL	5.4304 mL	13.5759 mL
	10 mM	0.2715 mL	1.3576 mL	2.7152 mL	6.7879 mL
	15 mM	0.1810 mL	0.9051 mL	1.8101 mL	4.5253 mL
	20 mM	0.1358 mL	0.6788 mL	1.3576 mL	3.3940 mL
	25 mM	0.1086 mL	0.5430 mL	1.0861 mL	2.7152 mL
	30 mM	0.0905 mL	0.4525 mL	0.9051 mL	2.2626 mL
	40 mM	0.0679 mL	0.3394 mL	0.6788 mL	1.6970 mL
	50 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3576 mL
	60 mM	0.0453 mL	0.2263 mL	0.4525 mL	1.1313 mL
	80 mM	0.0339 mL	0.1697 mL	0.3394 mL	0.8485 mL
	100 mM	0.0272 mL	0.1358 mL	0.2715 mL	0.6788 mL

VU591 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

VU5911222810-74-3VU 591VU-591Potassium ChannelKcsAROMK channelROMK InhibitorKir1.1neurologicalInhibitorinhibitorinhibit

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VU591

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Other Forms of VU591:

VU591 Related Antibodies

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VU591 Related Antibodies

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Complete Stock Solution Preparation Table

VU591 Related Classifications

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