2. PROTAC Apoptosis Epigenetics
  3. PROTACs Apoptosis METTL3
  4. WD6305 TFA

WD6305 TFA is a potent and selective METTL3-METTL14 PROTAC degrader. WD6305 TFA has DC50 values of 140 nM and 194 nM for METTL3 and METTL14, respectively. WD6305 TFA inhibits m6A modification and proliferation of AML cells, and induces apoptosis. WD6305 TFA has antitumor activity.

For research use only. We do not sell to patients.

WD6305 TFA Chemical Structure

WD6305 TFA Chemical Structure

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1 mg USD 218 In-stock
5 mg USD 545 In-stock
10 mg USD 905 In-stock
25 mg USD 1942 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of WD6305 TFA:

WD6305 TFA Related Antibodies

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Description

WD6305 TFA is a potent and selective METTL3-METTL14 PROTAC degrader. WD6305 TFA has DC50 values of 140 nM and 194 nM for METTL3 and METTL14, respectively. WD6305 TFA inhibits m6A modification and proliferation of AML cells, and induces apoptosis. WD6305 TFA has antitumor activity[1].

IC50 & Target

DC50: 140 nM (METTL3); D max: 91.9% (METTL3) [1]
DC50: 194 nM (METTL14)[1]
In Vitro

Non-salt dose:
WD6305 (20-5000 nM; 24 h) reduces METTL3 protein levels in Mono-Mac-6 cells with a DC50 of 140 nM and a D max of 91.9%[1].
WD6305 (20-5000 nM; 24 h) reduces METTL14 protein levels in Mono-Mac-6 cells with a DC50 of 194 nM[1].
WD6305 (0.5-10 μM; 48 h) decreases the ratio of m6A to polyA mRNA, inhibits cell proliferation and induces cell apoptosis in Mono-Mac-6 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WD6305 TFA Related Antibodies

Western Blot Analysis[1]

Cell Line: Mono-Mac-6 cells
Concentration: 20, 50, 100, 250, 500, 1000, 2000 and 5000 nM
Incubation Time: 24 h
Result: Reduced METTL3 and METTL14 protein levels in a dose-dependent manner.

Western Blot Analysis[1]

Cell Line: Mono-Mac-6 cells
Concentration: 1 and 2.5 μM
Incubation Time: 48 h
Result: Increased the levels of cleaved caspase-3 and cleaved PARP.
Molecular Weight

1226.40

Formula

C63H76F5N11O7S
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(N[C@@H](C(C)(C)C)C(N1[C@H](C(N[C@@H](C)C2=CC=C(C3=C(C)N=CS3)C=C2)=O)C[C@@H](O)C1)=O)CCC#CC(C=C4)=CC=C4CNC5=CC(N(CC6)CCC76NC(CN(C8=CC(F)=C(CN9CCC(C)(C)CC9)C=C8F)C7)=O)=NC=N5.O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 95 mg/mL (77.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8154 mL 4.0770 mL 8.1539 mL
5 mM 0.1631 mL 0.8154 mL 1.6308 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8154 mL 4.0770 mL 8.1539 mL 20.3849 mL
5 mM 0.1631 mL 0.8154 mL 1.6308 mL 4.0770 mL
10 mM 0.0815 mL 0.4077 mL 0.8154 mL 2.0385 mL
15 mM 0.0544 mL 0.2718 mL 0.5436 mL 1.3590 mL
20 mM 0.0408 mL 0.2038 mL 0.4077 mL 1.0192 mL
25 mM 0.0326 mL 0.1631 mL 0.3262 mL 0.8154 mL
30 mM 0.0272 mL 0.1359 mL 0.2718 mL 0.6795 mL
40 mM 0.0204 mL 0.1019 mL 0.2038 mL 0.5096 mL
50 mM 0.0163 mL 0.0815 mL 0.1631 mL 0.4077 mL
60 mM 0.0136 mL 0.0679 mL 0.1359 mL 0.3397 mL
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WD6305 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WD6305WD 6305WD-6305PROTACsApoptosisMETTL3Methyltransferase-like 3AMLMono-Mac-6 cellsInhibitorinhibitorinhibit

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