Werner syndrome RecQ helicase-IN-3

Name: Werner syndrome RecQ helicase-IN-3
Brand: MedChemExpress
SKU: HY-148740
Rating: 4.5 (1 reviews)

Cat. No.: HY-148740 Purity: 99.94%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Werner syndrome RecQ helicase-IN-3 is a potent and orally active werner syndrome recQ helicase (WRN) inhibitor with an IC₅₀ value of 0.06 µM. Werner syndrome RecQ helicase-IN-3 shows antiproliferative activity. Werner syndrome RecQ helicase-IN-3 shows anticancer activity.

For research use only. We do not sell to patients.

Werner syndrome RecQ helicase-IN-3 Chemical Structure

CAS No. : 2869954-98-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 937	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 937	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 620	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 992	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2088	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3333	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC₅₀: 0.06 µM (WRN)^[1]

In Vitro

Werner syndrome RecQ helicase-IN-3 (example 96) (0-10 µM; 8-20 days) shows antiproliferative activity with GI₅₀s of 0.06, >10 µM for SW48, DLD1 WRN-KO cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	SW48, DLD1 WRN-KO cells
Concentration:	0-10 µM
Incubation Time:	8-20 days
Result:	Showed antiproliferative activity with GI₅₀s of 0.06, >10 µM for SW48, DLD1 WRN-KO cells, respectively.

In Vivo

Werner syndrome RecQ helicase-IN-3 (120 mg/kg; daily for 22 days) shows anticancer activity in mouse^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Crl:NU(NCr)-Foxn1nu -Homozygous nude mice (SW48 xenografts cells)^[1]
Dosage:	120 mg/kg
Administration:	P.o.; daily for 22 days
Result:	Inhibited tumor growth.

Molecular Weight

687.07

Formula

C₃₁H₃₀ClF₃N₈O₅

CAS No.

2869954-98-1

Appearance

Solid

Color

White to off-white

SMILES

O=C(N1CCN(CC1)C2=C(CC)N(CC(NC3=CC=C(C(F)(F)F)C=C3Cl)=O)C4=NC(C5=CCOCC5)=NN4C2=O)C6=NC=CC=C6O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (90.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4555 mL	7.2773 mL	14.5546 mL
5 mM	0.2911 mL	1.4555 mL	2.9109 mL
10 mM	0.1455 mL	0.7277 mL	1.4555 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.03 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.94%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (254 KB) HNMR (316 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Vincent Bordas, et al. Triazolo-pyrimidine analogues for treating diseases connected to the inhibiton of werner syndrome recq helicase (wrn). WO2022249060A1.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.4555 mL	7.2773 mL	14.5546 mL	36.3864 mL
	5 mM	0.2911 mL	1.4555 mL	2.9109 mL	7.2773 mL
	10 mM	0.1455 mL	0.7277 mL	1.4555 mL	3.6386 mL
	15 mM	0.0970 mL	0.4852 mL	0.9703 mL	2.4258 mL
	20 mM	0.0728 mL	0.3639 mL	0.7277 mL	1.8193 mL
	25 mM	0.0582 mL	0.2911 mL	0.5822 mL	1.4555 mL
	30 mM	0.0485 mL	0.2426 mL	0.4852 mL	1.2129 mL
	40 mM	0.0364 mL	0.1819 mL	0.3639 mL	0.9097 mL
	50 mM	0.0291 mL	0.1455 mL	0.2911 mL	0.7277 mL
	60 mM	0.0243 mL	0.1213 mL	0.2426 mL	0.6064 mL
	80 mM	0.0182 mL	0.0910 mL	0.1819 mL	0.4548 mL