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  4. X-Gluc cyclohexanamine

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue).

For research use only. We do not sell to patients.

X-Gluc cyclohexanamine Chemical Structure

X-Gluc cyclohexanamine Chemical Structure

CAS No. : 114162-64-0

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10 mg USD 45 In-stock
25 mg USD 85 In-stock
50 mg USD 130 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of X-Gluc cyclohexanamine:

X-Gluc cyclohexanamine Related Antibodies

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  • Purity & Documentation

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Description

X-Gluc cyclohexanamine is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc cyclohexanamine can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc cyclohexanamine is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 20 mg X-Gluc cyclohexanamine in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.
2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.
3. Allow the plates to air dry and be used to inoculate the organisms.
4. Incubate the plates at 35°C and observe 16-24 h after inoculation.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

X-Gluc cyclohexanamine Related Antibodies
Molecular Weight

521.79

Formula

C20H26BrClN2O7
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1CCCCC1.O[C@H]([C@@H](O)[C@@H]2O)[C@@H](O[C@@H]2C(O)=O)OC3=CNC4=C3C(Cl)=C(Br)C=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (191.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9165 mL 9.5824 mL 19.1648 mL
5 mM 0.3833 mL 1.9165 mL 3.8330 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.96%

SDS (393 KB)

COA (254 KB) HNMR (124 KB) LCMS (240 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9165 mL 9.5824 mL 19.1648 mL 47.9120 mL
5 mM 0.3833 mL 1.9165 mL 3.8330 mL 9.5824 mL
10 mM 0.1916 mL 0.9582 mL 1.9165 mL 4.7912 mL
15 mM 0.1278 mL 0.6388 mL 1.2777 mL 3.1941 mL
20 mM 0.0958 mL 0.4791 mL 0.9582 mL 2.3956 mL
25 mM 0.0767 mL 0.3833 mL 0.7666 mL 1.9165 mL
30 mM 0.0639 mL 0.3194 mL 0.6388 mL 1.5971 mL
40 mM 0.0479 mL 0.2396 mL 0.4791 mL 1.1978 mL
50 mM 0.0383 mL 0.1916 mL 0.3833 mL 0.9582 mL
60 mM 0.0319 mL 0.1597 mL 0.3194 mL 0.7985 mL
80 mM 0.0240 mL 0.1198 mL 0.2396 mL 0.5989 mL
100 mM 0.0192 mL 0.0958 mL 0.1916 mL 0.4791 mL
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X-Gluc cyclohexanamine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

X-Gluc114162-64-0Fluorescent Dyeβ-glucuronidasedye reagentE. coliGUSmolecular biologyInhibitorinhibitorinhibit

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X-Gluc cyclohexanamine
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