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XTT (sodium) is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTT to a water-soluble, orange colored formazan product.

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XTT sodium Chemical Structure

XTT sodium Chemical Structure

CAS No. : 111072-31-2

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10 mM * 1 mL in DMSO
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XTT sodium Related Antibodies

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Description

XTT (sodium) is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTT to a water-soluble, orange colored formazan product.

In Vitro

Determining Cell Viability Using XTT (sodium)[2]
1. Solution Preparation
(1) XTT (sodium) solution:
Dissolve XTT in warm culture medium at a concentration of 1 mg/mL.
(2) PMS solution:
Prepare PMS solution at a concentration of 100 mM, dissolved in phosphate-buffered saline (PBS), and store at 4°C.
(3) Mixing for use:
When ready to use, add PMS solution to the XTT solution, resulting in a final PMS concentration of 25 μM.
2. Experimental Procedure
(1) Cell culture:
Seed the cells to be tested (e.g., HT-2 T cell line) in a 96-well plate, 100 μL medium per well, and incubate at 37°C for 40 hours.
(2) Add XTT/PMS solution:
Add 25 μL of the XTT/PMS solution to each well and incubate for 4-8 hours.
(3) Measure absorbance:
At the end of the incubation, measure the absorbance at 450 nm using a microplate reader, with 650 nm as the reference wavelength, and subtract the absorbance of the blank control wells.
3. Washing and Storage
Wash the experimental equipment with Milli-Q purified water. Store PMS and XTT solutions protected from light.
4. Notes
(1) XTT solution should be freshly prepared, and dissolving it in a 60°C water bath will be easier.
(2) The incubation time may vary depending on the experiment, and over-incubation can increase background noise.
(3) PMS solution has a short storage time and should be used within one month.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

XTT sodium Related Antibodies
Molecular Weight

673.52

Formula

C22H16N7NaO13S2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

COC1=C(N2N=C(N=[N+]2C3=CC(S([O-])(=O)=O)=C(C=C3OC)[N+]([O-])=O)C(NC4=CC=CC=C4)=O)C=C(S(=O)(O[Na])=O)C([N+]([O-])=O)=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (74.24 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4847 mL 7.4237 mL 14.8474 mL
5 mM 0.2969 mL 1.4847 mL 2.9695 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.71 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

COA (261 KB) HNMR (289 KB) LCMS (92 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4847 mL 7.4237 mL 14.8474 mL 37.1184 mL
5 mM 0.2969 mL 1.4847 mL 2.9695 mL 7.4237 mL
10 mM 0.1485 mL 0.7424 mL 1.4847 mL 3.7118 mL
15 mM 0.0990 mL 0.4949 mL 0.9898 mL 2.4746 mL
20 mM 0.0742 mL 0.3712 mL 0.7424 mL 1.8559 mL
25 mM 0.0594 mL 0.2969 mL 0.5939 mL 1.4847 mL
30 mM 0.0495 mL 0.2475 mL 0.4949 mL 1.2373 mL
40 mM 0.0371 mL 0.1856 mL 0.3712 mL 0.9280 mL
50 mM 0.0297 mL 0.1485 mL 0.2969 mL 0.7424 mL
60 mM 0.0247 mL 0.1237 mL 0.2475 mL 0.6186 mL
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XTT sodium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

XTT111072-31-2OthersInhibitorinhibitorinhibit

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