2. PI3K/Akt/mTOR
  3. Akt
  4. ZINC00784494

ZINC00784494 is a specific Lipocalin-2 (LCN2) inhibitor. ZINC00784494 inhibits cell proliferation, cell viability and reduces AKT phosphorylation levels in SUM149 cells. ZINC00784494 has good potential for research in inflammatory breast cancer (IBC).

For research use only. We do not sell to patients.

ZINC00784494 Chemical Structure

ZINC00784494 Chemical Structure

CAS No. : 317328-17-9

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1 mg USD 85 In-stock
5 mg USD 187 In-stock
10 mg USD 300 In-stock
25 mg USD 600 In-stock
50 mg USD 960 In-stock
100 mg USD 1530 In-stock
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Based on 1 publication(s) in Google Scholar

ZINC00784494 Related Antibodies

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Description

ZINC00784494 is a specific Lipocalin-2 (LCN2) inhibitor. ZINC00784494 inhibits cell proliferation, cell viability and reduces AKT phosphorylation levels in SUM149 cells. ZINC00784494 has good potential for research in inflammatory breast cancer (IBC)[1].

IC50 & Target

LCN2[1].
In Vitro

ZINC00784494 (0.01-100 µM; 72 h) reduces cell proliferation and cell viability in SUM149 cells[1].
ZINC00784494 (1, 10 µM; 15 min, 1 h) reduces the p-Akt levels in SUM149 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ZINC00784494 Related Antibodies

Cell Viability Assay[1]

Cell Line: SUM149 cells
Concentration: 0.01-100 µM
Incubation Time: 72 h
Result: Reduced cell viability at concentrations of 1 µM or lower.

Western Blot Analysis[1]

Cell Line: SUM149 cells
Concentration: 1, 10 µM
Incubation Time: 15 min, 1 h, 24 h
Result: Reduced the p-Akt protein levels 15 min and 1 h and changes in the p-Akt protein levels were not observed at 24 h.
Molecular Weight

362.40

Formula

C20H14N2O3S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(C2=CSC(NC3=CC=CC(C(C)=O)=C3)=N2)=CC4=CC=CC=C4O1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (275.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7594 mL 13.7969 mL 27.5938 mL
5 mM 0.5519 mL 2.7594 mL 5.5188 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (6.90 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7594 mL 13.7969 mL 27.5938 mL 68.9845 mL
5 mM 0.5519 mL 2.7594 mL 5.5188 mL 13.7969 mL
10 mM 0.2759 mL 1.3797 mL 2.7594 mL 6.8985 mL
15 mM 0.1840 mL 0.9198 mL 1.8396 mL 4.5990 mL
20 mM 0.1380 mL 0.6898 mL 1.3797 mL 3.4492 mL
25 mM 0.1104 mL 0.5519 mL 1.1038 mL 2.7594 mL
30 mM 0.0920 mL 0.4599 mL 0.9198 mL 2.2995 mL
40 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7246 mL
50 mM 0.0552 mL 0.2759 mL 0.5519 mL 1.3797 mL
60 mM 0.0460 mL 0.2299 mL 0.4599 mL 1.1497 mL
80 mM 0.0345 mL 0.1725 mL 0.3449 mL 0.8623 mL
100 mM 0.0276 mL 0.1380 mL 0.2759 mL 0.6898 mL
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ZINC00784494 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ZINC00784494317328-17-9ZINC 00784494ZINC-00784494AktPKBProtein kinase BIBCinflammatory breast cancerAKTproliferationSUM149 cellInhibitorinhibitorinhibit

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