β-Amanitin 

β-Amanitin is a cyclic peptide toxin in the poisonous Amanita phalloides mushroom. β-Amanitin inhibits inhibits eukaryotic RNA polymerase II and III. β-Amanitin inhibits protein synthesis. β-Amanitin can be used as a cytotoxic component of antibody-drug conjugates (ADCs)^[1][2].

β-Amanitin (0.01-100 μg/mL; 36 hours) shows toxicity in MCF-7 cells, and the rates of cell viability are calculated as 52%, 62%, 84%, 86%, and 91% at concentrations of 100, 10, 1, 0.1, and 0.01 μg/mL, respectively^[2].
β-Amanitin shows a great inhibition of protein synthesis at both concentrations (10 μg/mL and 1 μg/mL) in MCF-7 cells for 24 hours^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

919.95

C₃₉H₅₃N₉O₁₅S

21150-22-1

Solid

White to off-white

OC1=CC=C(C2=C1)C(C[C@]3([H])NC([C@](NC([C@@]4([H])N(C[C@H](O)C4)C([C@@](CC(O)=O)([H])NC([C@](NC(CNC([C@@]([C@@H](C)CC)([H])NC(CNC3=O)=O)=O)=O)([H])C5)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)=O)=C(N2)S5=O

Room temperature in continental US; may vary elsewhere.

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Kaya E, et al. Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line. Turk J Med Sci. 2014;44(5):728-32.  [Content Brief]

  [2]. Lutz C, et al. Alpha- and Beta-Amanitin Total Synthesis. Angew Chem Int Ed Engl. 2020 Feb 24.  [Content Brief]