β-Amyrin acetate

Name: β-Amyrin acetate
Brand: MedChemExpress
SKU: HY-N2923
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2923 Purity: 99.47%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

β-Amyrin acetate is a triterpenoid with potent anti-inflammatory, antifungal, anti-diabetic, anti-hyperlipidemic activities. β-Amyrin acetate can inhibit HMG-CoA reductase activity by locating in the hydrophobic binding cleft of HMG CoA reductase.

For research use only. We do not sell to patients.

β-Amyrin acetate Chemical Structure

CAS No. : 1616-93-9

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	11.4 μM Compound: 2	Growth inhibition of human A549 cells by MTT assay Growth inhibition of human A549 cells by MTT assay	[PMID: 20153184]
BMDC	IC₅₀	> 50 μM Compound: 23	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL-12p40 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	> 50 μM Compound: 23	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated IL12 production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
BMDC	IC₅₀	> 50 μM Compound: 23	Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA Antiinflammatory activity in C57BL/6 mouse BMDCs assessed as inhibition of LPS-stimulated TNF-alpha production treated 1 hr before LPS challenge measured 18 hrs post stimulation by ELISA	[PMID: 23484668]
HeLa	IC₅₀	> 50 μM Compound: 3	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19447618]
HL-60	GI₅₀	14.6 μM Compound: 2	Growth inhibition of human HL60 cells by MTT assay Growth inhibition of human HL60 cells by MTT assay	[PMID: 20153184]
HT-29	GI₅₀	12.5 μM Compound: 2	Growth inhibition of human HT-29 cells by MTT assay Growth inhibition of human HT-29 cells by MTT assay	[PMID: 20153184]
MCF7	IC₅₀	26.73 μM Compound: 3	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19447618]
SK-MEL-1	IC₅₀	> 50 μM Compound: 3	Cytotoxicity against human SK-MEL-1 cells by MTT assay Cytotoxicity against human SK-MEL-1 cells by MTT assay	[PMID: 19447618]
SK-OV-3	GI₅₀	14.6 μM Compound: 2	Growth inhibition of human SKOV3 cells by MTT assay Growth inhibition of human SKOV3 cells by MTT assay	[PMID: 20153184]

In Vitro

β-Amyrin acetate (50 μg/mL) inhibits heat-induced hemolysis and hypotonicity-induced hemolysis of human erythrocytes^[1].
β-Amyrin acetate (5-100 μM) has HMG-CoA reductase inhibitory activity by locating in the hydrophobic binding cleft lined with residues Leu562, Gly560, Ala564, Gly569, Ser852, Leu853, Leu857, Met854, Ala856, Ser852 and Ala855 of human HMG CoA reductase^[2].
β-Amyrin acetate (7.8-1000 μg/mL, 48 h) inhibits all of the Candida fungal species tested with MIC values ranging from 30 to 250 μg/mL)^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

β-Amyrin acetate (applied on the anterior surface of the right ear, 100 μg/ear, a single dose) significantly inhibits xylene-induced ear edema in mice^[1].
β-Amyrin acetate (intraperitoneal injection, 4 mg/100 g, daily for 6 consecutive days) shows significant anti-inflammatory activities (43.6%) in adult albino rats^[3].
β-Amyrin acetate (subcutaneous injection, 4 mg/100 g, daily for 10 days ) increases the ATP-phosphohydrolase activity in liver homogenates both in normal and arthritic rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xylene-induced mouse ear topical edema model^[1]
Dosage:	50 and 100 μg/ear (5 μL)
Administration:	Applied on the anterior surface of the right ear
Result:	Inhibited Xylene-induced ear edema in mice

Animal Model:	Adult albino rats^[3]
Dosage:	4 mg/100g, daily for 6 consecutive days
Administration:	Intraperitoneal injection
Result:	Showed significant anti-inflammatory activities with mean average weight of granulation tissue of 9.2 mg after 6 days.

Molecular Weight

468.75

Formula

C₃₂H₅₂O₂

CAS No.

1616-93-9

Appearance

Solid

Color

White to off-white

SMILES

C[C@]12[C@@](CC=C3[C@]2(CC[C@@]4([C@@]3([H])CC(C)(CC4)C)C)C)([H])[C@@]5([C@@](C(C)([C@H](CC5)OC(C)=O)C)([H])CC1)C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

Ethanol : 1 mg/mL (2.13 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1333 mL	10.6667 mL	21.3333 mL
5 mM	---	---	---
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.1 mg/mL (0.21 mM); Clear solution

This protocol yields a clear solution of ≥ 0.1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (1.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.1 mg/mL (0.21 mM); Clear solution

This protocol yields a clear solution of ≥ 0.1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (1.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.47%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (252 KB) HNMR (237 KB) RP-HPLC (306 KB)

Handling Instructions (2659 KB)

References

[1]. Nkeoma Nkasi Okoye, et al. beta-Amyrin and alpha-amyrin acetate isolated from the stem bark of Alstonia boonei display profound anti-inflammatory activity. Pharm Biol. 2014 Nov;52(11):1478-86. [Content Brief]

[2]. Ranjani Maurya, et al. β-Amyrin acetate and β-amyrin palmitate as antidyslipidemic agents from Wrightia tomentosa leaves. Phytomedicine. 2012 Jun 15;19(8-9):682-5. [Content Brief]

[3]. M.B.Gupta, et al. Biochemical study of the anti-inflammatory activity of α and β-amyrin acetate. Biochemical Pharmacology. 1971 Feb; 2(20): 401-405.

[4]. S Johann, et al. Antifungal activity of the amyrin derivatives and in vitro inhibition of Candida albicans adhesion to human epithelial cells. Lett Appl Microbiol. 2007 Aug;45(2):148-53. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol

1 mM

2.1333 mL

10.6667 mL

21.3333 mL

53.3333 mL