(10E,12Z)-Octadeca-10,12-dienoic acid (Synonyms: trans-10,cis-12 CLA2)

Cat. No.: HY-116538 Purity: 99.10%: FAQs
Data Sheet SDS COA Handling Instructions

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(10E,12Z)-Octadeca-10,12-dienoic acidactivates PPAR α and inhibits adipocyte differentiation. (10E,12Z)-Octadeca-10,12-dienoic acid and its downstream metabolites have various antioxidant and antitumor activities. (10E,12Z)-Octadeca-10,12-dienoic acid is effective orally.

For research use only. We do not sell to patients.

(10E,12Z)-Octadeca-10,12-dienoic acid Chemical Structure

CAS No. : 2420-56-6

Size	Price	Stock
Solvent
5 mg (89.14 mM * 200 μL in Ethanol)	USD 70	Ask For Quote & Lead Time
Solvent
10 mg (89.14 mM * 400 μL in Ethanol)	USD 110	Ask For Quote & Lead Time

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This product is a controlled substance and not for sale in your territory.

Other Forms of (10E,12Z)-Octadeca-10,12-dienoic acid:

(10E,12Z)-Octadeca-10,12-dienoic acid (Standard) Get quote

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PPARα PPARβ/δ PPARγ PPAR PPARδ

Biological Activity
Purity & Documentation
References
Customer Review

Description

(10E,12Z)-Octadeca-10,12-dienoic acidactivates PPAR α and inhibits adipocyte differentiation^[1]. (10E,12Z)-Octadeca-10,12-dienoic acid and its downstream metabolites have various antioxidant and antitumor activities. (10E,12Z)-Octadeca-10,12-dienoic acid is effective orally^[2]^[3].

IC₅₀ & Target^[1]

PPARα

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	15 μM Compound: 8	Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique	[PMID: 20176483]

Molecular Weight

280.45

Formula

C₁₈H₃₂O₂

CAS No.

2420-56-6

Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C=C\CCCCCCCCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (178.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.10%

Select Batch:

Data Sheet (266 KB) SDS (393 KB)

COA (238 KB)

Handling Instructions (2659 KB)

References

[1]. Houseknecht KL, et al. Dietary conjugated linoleic acid normalizes impaired glucose tolerance in the Zucker diabetic fatty fa/fa rat. Biochem Biophys Res Commun. 1998 Mar 27;244(3):678-82. [Content Brief]

[2]. Shultz TD, et al.Inhibitory effect of conjugated dienoic derivatives of linoleic acid and beta-carotene on the in vitro growth of human cancer cells. Cancer Lett. 1992 Apr 15;63(2):125-33. [Content Brief]

[3]. Park Y, et al Conjugated fatty acids as a prevention tool for obesity and osteoporosis[M]//Emerging trends in dietary components for preventing and combating disease. American Chemical Society, 2012: 393-405

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5657 mL	17.8285 mL	35.6570 mL	89.1424 mL
	5 mM	0.7131 mL	3.5657 mL	7.1314 mL	17.8285 mL
	10 mM	0.3566 mL	1.7828 mL	3.5657 mL	8.9142 mL
	15 mM	0.2377 mL	1.1886 mL	2.3771 mL	5.9428 mL
	20 mM	0.1783 mL	0.8914 mL	1.7828 mL	4.4571 mL
	25 mM	0.1426 mL	0.7131 mL	1.4263 mL	3.5657 mL
	30 mM	0.1189 mL	0.5943 mL	1.1886 mL	2.9714 mL
	40 mM	0.0891 mL	0.4457 mL	0.8914 mL	2.2286 mL
	50 mM	0.0713 mL	0.3566 mL	0.7131 mL	1.7828 mL
	60 mM	0.0594 mL	0.2971 mL	0.5943 mL	1.4857 mL
	80 mM	0.0446 mL	0.2229 mL	0.4457 mL	1.1143 mL
	100 mM	0.0357 mL	0.1783 mL	0.3566 mL	0.8914 mL

(10E,12Z)-Octadeca-10,12-dienoic acid Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(10E,12Z)-Octadeca-10,12-dienoic acid2420-56-6trans-10,cis-12 CLA2Endogenous MetabolitePPARPeroxisome proliferator-activated receptorsFat BurnerMetabolism BoosterDietary SupplementOmega-6 Fatty AcidAnti-InflammatoryAntioxidantInhibitorinhibitorinhibit

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