1. Metabolic Enzyme/Protease Vitamin D Related/Nuclear Receptor Cell Cycle/DNA Damage
  2. Endogenous Metabolite PPAR
  3. (10E,12Z)-Octadeca-10,12-dienoic acid

(10E,12Z)-Octadeca-10,12-dienoic acid  (Synonyms: trans-10,cis-12 CLA2)

Cat. No.: HY-116538 Purity: 99.10%
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(10E,12Z)-Octadeca-10,12-dienoic acidactivates PPAR α and inhibits adipocyte differentiation. (10E,12Z)-Octadeca-10,12-dienoic acid and its downstream metabolites have various antioxidant and antitumor activities. (10E,12Z)-Octadeca-10,12-dienoic acid is effective orally.

For research use only. We do not sell to patients.

(10E,12Z)-Octadeca-10,12-dienoic acid Chemical Structure

(10E,12Z)-Octadeca-10,12-dienoic acid Chemical Structure

CAS No. : 2420-56-6

Size Price Stock
Solvent
5 mg (89.14 mM * 200 μL in Ethanol) Ask For Quote & Lead Time
Solvent
10 mg (89.14 mM * 400 μL in Ethanol) Ask For Quote & Lead Time
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Other Forms of (10E,12Z)-Octadeca-10,12-dienoic acid:

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Description

(10E,12Z)-Octadeca-10,12-dienoic acidactivates PPAR α and inhibits adipocyte differentiation[1]. (10E,12Z)-Octadeca-10,12-dienoic acid and its downstream metabolites have various antioxidant and antitumor activities. (10E,12Z)-Octadeca-10,12-dienoic acid is effective orally[2][3].

IC50 & Target[1]

PPARα

 

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
15 μM
Compound: 8
Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique
Inhibition of CRM1 interaction with HIV1 HA-tagged Rev in HeLa cells assessed as inhibition of Rev nuclear export by indirect fluorescent antibody technique
[PMID: 20176483]
Molecular Weight

280.45

Formula

C18H32O2

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C=C\CCCCCCCCC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility
In Vitro: 

DMSO : ≥ 50 mg/mL (178.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (8.91 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.10%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5657 mL 17.8285 mL 35.6570 mL 89.1424 mL
5 mM 0.7131 mL 3.5657 mL 7.1314 mL 17.8285 mL
10 mM 0.3566 mL 1.7828 mL 3.5657 mL 8.9142 mL
15 mM 0.2377 mL 1.1886 mL 2.3771 mL 5.9428 mL
20 mM 0.1783 mL 0.8914 mL 1.7828 mL 4.4571 mL
25 mM 0.1426 mL 0.7131 mL 1.4263 mL 3.5657 mL
30 mM 0.1189 mL 0.5943 mL 1.1886 mL 2.9714 mL
40 mM 0.0891 mL 0.4457 mL 0.8914 mL 2.2286 mL
50 mM 0.0713 mL 0.3566 mL 0.7131 mL 1.7828 mL
60 mM 0.0594 mL 0.2971 mL 0.5943 mL 1.4857 mL
80 mM 0.0446 mL 0.2229 mL 0.4457 mL 1.1143 mL
100 mM 0.0357 mL 0.1783 mL 0.3566 mL 0.8914 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
(10E,12Z)-Octadeca-10,12-dienoic acid
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HY-116538
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