2,5-Di-tert-butylhydroquinone

Name: 2,5-Di-tert-butylhydroquinone
Brand: MedChemExpress
SKU: HY-W012399
Rating: 4.5 (1 reviews)

Cat. No.: HY-W012399 Purity: 99.90%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2,5-Di-tert-butylhydroquinone (DTBHQ), the indirect food additive, regulates the activity of 5-lipoxygenase as well as the activity of COX-2 (IC₅₀=1.8 and 14.1 μM for 5-LO and COX-2, respectively) .

For research use only. We do not sell to patients.

2,5-Di-tert-butylhydroquinone Chemical Structure

CAS No. : 88-58-4

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 33	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: December 31
Solid
25 g	USD 30	In-stock	Estimated Time of Arrival: December 31
50 g	USD 40	In-stock	Estimated Time of Arrival: December 31
> 100 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Lipoxygenase Isoform Specific Products:

View All Isoforms

5-LOX 12-LOX 15-LOX

View All COX Isoform Specific Products:

View All Isoforms

COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

5-LO

1.8 μM (IC₅₀)

COX-2

14.1 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Sf9	IC₅₀	0.13 μM Compound: BHQ	Inhibition of Heliothis virescens SERCA expressed in Sf9 cells assessed as ATP depletion measured after 60 mins by luciferin/luciferase coupled reporter gene assay Inhibition of Heliothis virescens SERCA expressed in Sf9 cells assessed as ATP depletion measured after 60 mins by luciferin/luciferase coupled reporter gene assay	[PMID: 21719297]

In Vitro

DTBHQ influences arachidonic acid (AA) metabolism in biologically relevant concentrations in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

222.32

Formula

C₁₄H₂₂O₂

CAS No.

88-58-4

Appearance

Solid

Color

White to off-white

SMILES

OC1=CC(C(C)(C)C)=C(O)C=C1C(C)(C)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (449.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4980 mL	22.4901 mL	44.9802 mL
5 mM	0.8996 mL	4.4980 mL	8.9960 mL
10 mM	0.4498 mL	2.2490 mL	4.4980 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (11.25 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.90%

Select Batch:

Data Sheet (274 KB) SDS (625 KB)

COA (251 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Zsofia Kutil, et al. The influence of the quinone antioxidants tert-butylhydroquinone and 2,5-di-tert-butylhydroquinone on the arachidonic acid metabolism in vitro. Food and Agricultural Immunology. Volume 26, 2015-Issue 4.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4980 mL	22.4901 mL	44.9802 mL	112.4505 mL
	5 mM	0.8996 mL	4.4980 mL	8.9960 mL	22.4901 mL
	10 mM	0.4498 mL	2.2490 mL	4.4980 mL	11.2451 mL
	15 mM	0.2999 mL	1.4993 mL	2.9987 mL	7.4967 mL
	20 mM	0.2249 mL	1.1245 mL	2.2490 mL	5.6225 mL
	25 mM	0.1799 mL	0.8996 mL	1.7992 mL	4.4980 mL
	30 mM	0.1499 mL	0.7497 mL	1.4993 mL	3.7484 mL
	40 mM	0.1125 mL	0.5623 mL	1.1245 mL	2.8113 mL
	50 mM	0.0900 mL	0.4498 mL	0.8996 mL	2.2490 mL
	60 mM	0.0750 mL	0.3748 mL	0.7497 mL	1.8742 mL
	80 mM	0.0562 mL	0.2811 mL	0.5623 mL	1.4056 mL
	100 mM	0.0450 mL	0.2249 mL	0.4498 mL	1.1245 mL