2-(2-Methylbenzamido)acetic acid

Name: 2-(2-Methylbenzamido)acetic acid
Brand: MedChemExpress
SKU: HY-W015060
Rating: 4.5 (1 reviews)

Cat. No.: HY-W015060 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

2-(2-Methylbenzamido)acetic acid is a metabolite detected in urine.

For research use only. We do not sell to patients.

2-(2-Methylbenzamido)acetic acid Chemical Structure

CAS No. : 42013-20-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
5 g		Get quote
10 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of 2-(2-Methylbenzamido)acetic acid:

2-(2-Methylbenzamido)acetic acid-d₇ Get quote
2-(2-Methylbenzamido)acetic acid-d₂ Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

2-(2-Methylbenzamido)acetic acid is a metabolite detected in urine.

IC₅₀ & Target

Human Endogenous Metabolite

Molecular Weight

193.20

Formula

C₁₀H₁₁NO₃

CAS No.

42013-20-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(O)CNC(C1=CC=CC=C1C)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (517.60 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1760 mL	25.8799 mL	51.7598 mL
5 mM	1.0352 mL	5.1760 mL	10.3520 mL
10 mM	0.5176 mL	2.5880 mL	5.1760 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (270 KB) SDS (393 KB)

COA (246 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Remane D, et al. Validation of a multi-analyte HPLC-DAD method for determination of uric acid, creatinine, homovanillic acid, niacinamide, hippuric acid, indole-3-acetic acid and 2-methylhippuric acid in human urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Aug 15;998-999:40-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1760 mL	25.8799 mL	51.7598 mL	129.3996 mL
	5 mM	1.0352 mL	5.1760 mL	10.3520 mL	25.8799 mL
	10 mM	0.5176 mL	2.5880 mL	5.1760 mL	12.9400 mL
	15 mM	0.3451 mL	1.7253 mL	3.4507 mL	8.6266 mL
	20 mM	0.2588 mL	1.2940 mL	2.5880 mL	6.4700 mL
	25 mM	0.2070 mL	1.0352 mL	2.0704 mL	5.1760 mL
	30 mM	0.1725 mL	0.8627 mL	1.7253 mL	4.3133 mL
	40 mM	0.1294 mL	0.6470 mL	1.2940 mL	3.2350 mL
	50 mM	0.1035 mL	0.5176 mL	1.0352 mL	2.5880 mL
	60 mM	0.0863 mL	0.4313 mL	0.8627 mL	2.1567 mL
	80 mM	0.0647 mL	0.3235 mL	0.6470 mL	1.6175 mL
	100 mM	0.0518 mL	0.2588 mL	0.5176 mL	1.2940 mL