2-Chlorohexadecanoic acid

Name: 2-Chlorohexadecanoic acid
Brand: MedChemExpress
SKU: HY-131688
Rating: 4.5 (1 reviews)

Cat. No.: HY-131688 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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2-Chlorohexadecanoic acid, an inflammatory lipid mediator, interferes with protein palmitoylation,induces ER-stress markers, reduced the ER ATP content, and activates transcription and secretion of IL-6 as well as IL-8.2-Chlorohexadecanoic acid disrupts the mitochondrial membrane potential and induces procaspase-3 and PARP cleavage.2-Chlorohexadecanoic acid can across blood-brain barrier (BBB) and compromises ER- and mitochondrial functions in the human brain endothelial cell line hCMEC/D3.

For research use only. We do not sell to patients.

2-Chlorohexadecanoic acid Chemical Structure

CAS No. : 19117-92-1

Size	Price	Stock	Quantity
1 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 520	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1100	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

In Vitro

2-Chlorohexadecanoic acid (2-ClHA; 10 μM; 4, 6 h) results phosphorylation of eIF2α starting 4 h post treatment, while total eIF2α levels remained unchanged incubation of hCMEC/D3 cells. 2-Chlorohexadecanoic acid increases expression of ATF4, a target gene of eIF2α^[1].
2-Chlorohexadecanoic acid (25 µM, 30 min) induces a decrease in the FRET ratio signal of ERAT by 40%, indicating significantly diminished [ATP]ER in cells that were treated with 2-Chlorohexadecanoic acid^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

290.87

Formula

C₁₆H₃₁ClO₂

CAS No.

19117-92-1

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCCCC(Cl)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (68.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.4380 mL	17.1898 mL	34.3796 mL
5 mM	0.6876 mL	3.4380 mL	6.8759 mL
10 mM	0.3438 mL	1.7190 mL	3.4380 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2 mg/mL (6.88 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2 mg/mL (6.88 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (266 KB) SDS (251 KB)

COA (239 KB) HNMR (157 KB) RP-HPLC (278 KB) LCMS (389 KB)

Handling Instructions (2659 KB)

References

[1]. Eva Bernhart, et al. 2-Chlorohexadecanoic acid induces ER stress and mitochondrial dysfunction in brain microvascular endothelial cells. Redox Biol. 2018 May;15:441-451. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.4380 mL	17.1898 mL	34.3796 mL	85.9491 mL
	5 mM	0.6876 mL	3.4380 mL	6.8759 mL	17.1898 mL
	10 mM	0.3438 mL	1.7190 mL	3.4380 mL	8.5949 mL
	15 mM	0.2292 mL	1.1460 mL	2.2920 mL	5.7299 mL
	20 mM	0.1719 mL	0.8595 mL	1.7190 mL	4.2975 mL
	25 mM	0.1375 mL	0.6876 mL	1.3752 mL	3.4380 mL
	30 mM	0.1146 mL	0.5730 mL	1.1460 mL	2.8650 mL
	40 mM	0.0859 mL	0.4297 mL	0.8595 mL	2.1487 mL
	50 mM	0.0688 mL	0.3438 mL	0.6876 mL	1.7190 mL
	60 mM	0.0573 mL	0.2865 mL	0.5730 mL	1.4325 mL